Genetic diversity and gene expression of drought tolerance Mulberry in Thailand

Name: Nutdanai Putthisawong

LCSH: Kasetsart University -- Dissertations. Ph.D. (Genetics) 2024.

Classification :.LCCS: QK495.M73

LCSH: Kasetsart University. -- Department of Genetics -- Dissertations.

LCSH: Mulberry -- Genetics.

LCSH: Mulberry -- Drought tolerance.

LCSH: Mulberry -- Thailand.

LCSH: Polyploidy.

LCSH: Genetic markers.

LCSH: Gene expression.

Abstract: The genus Morus commonly referred to as mulberry, is a vital dietary source essential to the sericulture industry. Environmental factors play a crucial role in determining the yield of mulberry leaves, with water scarcity, particularly drought, being one of the most significant influencers. The study was to investigate the genetic variability present in 85 reserved mulberry accessions from the mulberry germplasm repository at the Queen Sirikit Sericulture Centre (Sisaket), Thailand. This was achieved through the utilization of Sequence-Related Amplified Polymorphism (SRAP) and Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) markers, in tandem with the analysis of morphological characteristics. Additionally, the study involved examining the gene expression patterns of selected mulberry varieties with different ploidy levels, specifically “KhunPai” and “KhunPai4X”, under drought conditions, employing the RNA-Seq technique. The study successfully identified four species within Thai mulberry namely M. alba, M. australis, M. macroura, and M. rubra, along with one accession that remains unidentified based on morphological characteristics. These species were classified into six distinct groups viz wild mulberry, wild hybrid mulberry, Thai native mulberry, Thai fruit mulberry, foreign mulberry, and breeding mulberry. The application of 12 combination primers for SRAP and 11 primers designed for newly developed EST-SSR markers, along with morphological traits, clearly established the relationship between groups and species of Thai mulberry. The impact of drought stress on Thai mulberry was examined over an 8-day period, with a specific focus on discerning phenotypic disparities between diploid “KhunPai” and mixoploid “KhunPai 4X” plants. Comparative analysis of gene expression profiles revealed a notable difference of 2.67% between diploid and mixoploid plants. Remarkably, mixoploid plants displayed a greater abundance of overexpressed genes linked to enhanced drought tolerance under such conditions, in contrast to diploid counterparts. Furthermore, functional annotation of these differentially expressed genes unveiled the enrichment of 17 KEGG pathways consistently observed across both experimental conditions and all time points. Notably, mixoploid mulberry demonstrated a heightened gene expression within a pivotal pathway compared to diploid mulberry.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chatchawan Jantasuriyarat

Role: Thesis Advisor

Name: Chatuporn Kuleung

Role: Thesis Co-Advisor

Name: Weerasin Sonjaroon

Role: Thesis Co-Advisor

Created: 2024

Modified: 2026-07-08

Issued: 2026-07-08

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2566/nutdanai-put-all.pdf

CallNumber: QK495.M73 .N88

eng

Spatial: Thailand.

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Genetics

Grantor: Kasetsart University

©copyrights Kasetsart University

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