Electrochemical DNA biosensor for MPOX virus detection

Name: Piyathida Phimdee

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Lalitphan Hongtanee

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Sudkate Chaiyo

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Name: Yakoh, Abdulhadee

Organization : Chulalongkorn University. The Institute of Biotechnology and Genetic Engineering

Email : Abdulhadee.y@chula.ac.th

LCSH: Monkeypox virus -- Detection

LCSH: Mpox (Disease) -- Detection

LCSH: Biosensors

LCSH: Nucleic acids

Abstract: In 2024, the resurgence of MPOX cases prompted the World Health Organization (WHO) to maintain heightened surveillance and diagnostic readiness. Although polymerase chain reaction (PCR) remains the gold standard for MPOX diagnosis, its high cost and lengthy processing time limit accessibility, particularly in low-resource settings. To address these challenges, this study developed a label-free electrochemical nucleic acid nanobiosensor for the detection of the MPOX virus (MPXV) without the need for conventional tagging or target amplification. The sensor design utilizes the mismatched nucleic acid–metal ion (MNM) nanocomplex principle, wherein a DNA probe containing a deliberate cytosine–cytosine (C–C) mismatch hybridizes with the wild-type MPXV DNA target. Silver ions (Ag⁺) selectively intercalate at the mismatched site, forming stable coordinate covalent bonds. The distinctive redox behavior and rapid intercalation of Ag⁺ enable highly sensitive electrochemical detection. Experimental optimization of DNA probe concentration, hybridization time, Ag⁺ concentration, and incubation time revealed that the electrical response increased proportionally with target DNA concentration, achieving a limit of detection (LOD) of 2 pM. This biosensor represents a significant advancement in MPXV detection technology, providing a rapid, cost-effective, and highly sensitive diagnostic platform suitable for point-of-care testing and outbreak management

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2025

Modified: 2025-10-22

Issued: 2025-10-22

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BibliograpyCitation : In The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana and Suranaree University of Technology. Institute of Science. School of Chemistry. Pure and Applied Chemistry International Conference 2025 (PACCON 2025) (pp.42-47). Bangkok : The Chemical Society of Thailand under the Patronage of Professor Dr. Her Royal Highness Princess Chulabhorn Krom Phra Srisavangavadhana in association with the School of Chemistry, Institute of Science, Suranaree University of Technology, 2025

eng

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