Effect of organic trace mineral on function of oxidative stress and meat quality responsible enzyme in young chick

Name: Thanapol Puangmalee

LCSH: Kasetsart University -- Theses. M.S. (Agricultural Biotechnology) 2020

Classification :.LCCS: SF498.7

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Theses

LCSH: Broilers (Chickens) -- Feeding and feeds)

LCSH: Broilers (Chickens) -- Effect of stress on)

LCSH: Chickens

LCSH: Enzyme

Abstract: Trace minerals play every role that associated with metabolism process and necessary for the growth of animals. In general, mineral premixes are produced in salts form of inorganic minerals to full fill mineral requirement of animal. However inorganic minerals are relatively low availability in broilers. The organic mineral form has higher availability than the inorganic minerals. It can be used in lower doses without affecting production performance of broilers. This research is aimed to investigate the effects of organic mineral as a total replace to inorganic mineral on growth performance, antioxidant function and meat quality responsible enzyme on gene expression by using real-time PCR. Eight hundred, one day old, male broiler chicks of Ross 308 were divided into 4 dietary treatments including: T1) Positive control (PC) Diet with inorganic premix, T2) PC + organic minerals 500 g/tonne, T3) diet without inorganic Zn Cu Mn Se I and Fe in premix + organic minerals 750 g/tonne and T4) T3 + selenium yeast 200 g/ton. The trail ran between 1 and 21 days of age. Growth performance no significant differences between treatments in terms of feed intake and mortality. In body weight gain and ADG, T2 and T1 not differences but higher than T3 and T4 (P<0.05) affected same the result of feed conversion ratio. The experiments on gene expression, muscle and liver samples immediately collected and perform to quantify the mRNA expression level of gene involved in meat quality and antioxidant function by real-time PCR. Supplementation of broiler diets with organic form of mineral was increased mRNA expression of a gene involved the glutathione redox cycle including glutathione peroxidase and glutathione S-transferase-α (P<0.05). Furthermore, organic trace mineral was decreased mRNA expression of a gene involved in meat quality to show that there is an increase in muscle development (P<0.05). Blood sample for Antioxidant enzyme measurement was selected form RNA expression measurement. GPx enzyme was measured by enzyme-linked immune sorbent assay (ELISA) kit. The concentration of glutathione peroxidase in antioxidant enzyme measurement was increased same with the result of mRNA expression (P<0.05). Thus, replacement of organic trace mineral supplements in broiler diets may be improve growth rate and meat quality for trace mineral supplementation of feed mixtures for poultry.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Wirawan Junlapho

Role: Thesis Advisor

Name: Yuwares Ruangpanit

Role: Thesis CO-Advisor

Created: 2020

Modified: 2025-08-03

Issued: 2025-08-03

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2563/thanapol-pua-all.pdf

CallNumber: SF498.7 .T44

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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