Phytochemical analysis and biological activities of antidesma thwaitesianum fruit extracts

Name: Soukbandith Pheungsomphane

LCSH: Kasetsart University -- Theses. M.S. (Food Technology) 2019

Classification :.LCCS: QK495.E9

LCSH: Kasetsart University. -- Department of Food Technology and Nutrition -- Theses

LCSH: Antidesma -- Utilization

LCSH: Phytochemicals

LCSH: Plant extracts

LCSH: Bioactive compounds

LCSH: Foodborne diseases -- Microbiology

LCSH: Anti-infective agents

Abstract: Antidesma thwaitesianum Müll. Arg. (Mao-Luang) fruits were extracted with various ethanol concentrations. The highest total phenolic content was obtained by using 50 % ethanol. The different maturity fruit (green, red and black) extracts were analyzed for total phenolic content and antimicrobial activities. Green fruits showed the highest total phenolic content. However, all different maturity fruit extracts exhibited antimicrobial activity against Bacillus cereus and Escherichia coli. Only ripened black fruit extract could inhibit Aspergillus flavus. Ripened fruits were extracted using different extraction methods: squeeze, ethanol extraction, and decoction. Mao-Luang juice (MJ), marc and seed ethanol extracts (MME and MSE, respectively), and marc and seed decoction (MMD and MSD, respectively) were obtained. MSD revealed the highest total phenolic content, while the high total flavonoid content was found in MJ, MME, and MSE. MSE had strong scavenging activity of both DPPH and ABTS+ free radicals, while MJ and MSD exerted moderate activity in reducing ferric ions. All crude extracts were potentially effective with variable efficiency against B. cereus, Listeria monocytogenes, Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, and Salmonella Typhimurium. Moreover, MJ had an inhibitory effect against A. flavus and Penicillium digitatum. However, bactericidal activity against B. cereus and L. monocytogenes was observed only in marc extracts (MME and MMD). Bioactive compounds in MME and MMD were separated using liquid-liquid extraction and analyzed by HPLC UV-Visible. Phenolic acids (caffeic, chlorogenic, ferulic, gallic, and vanillic acid) and flavonoids (catechin, myricetin, quercetin, and rutin) were isolated from ethyl acetate and n-butanol fractions with different concentrations. Most phenolic acids and flavonoids found in extracts, except for rutin, had antimicrobial activity with different minimum inhibitory concentrations. These findings support the potential use of Mao-Luang as an antimicrobial agent and commercial health products.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chintana Tayuan

Role: Thesis Advisor

Name: Patcharaporn Tinchan

Role: Thesis CO-Advisor

Created: 2019

Issued: 2025-08-03

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: QK495.E9 .S68

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Food Technology

Grantor: Kasetsart University

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