Effects of crocodile (Crocodylus siamensis) oil extracts on liver detoxification enzymes and lipid metabolism in rats fed a high-fat diet

Name: Pitchaya Santativongchai

LCSH: Kasetsart University -- Dissertations. Ph.D. (Bio-Veterinary Sciences) 2022

Classification :.LCCS: TP676 .P58

LCSH: Kasetsart University. -- Faculty of Veterinary Medicine -- Dissertations

LCSH: Crocodile oil

LCSH: Omega-3 fatty acids

LCSH: Fatty acids

LCSH: Peroxides

LCSH: Palm Oil

LCSH: Leptin

LCSH: Enzyme-linked immunosorbent assay

LCSH: Immunohistochemistry

LCSH: Cytochrome P-450

Abstract: Crocodile oil has been used worldwide for cosmetic and pharmaceutical purposes due to its essential fatty acid contents. However, the other benefits and the involved molecular mechanisms of the oil have been investigated with limited data. This study aimed to investigate the physicochemical properties of crocodile oil obtained from Siamese crocodile (Crocodylus siamensis) and to determine the effects of the oil on serum lipid parameters, leptin signaling, and the activities of detoxification enzymes in rats fed a high-fat diet. The male Wistar rats (n = 7/group) were divided into control, palm oil, and crocodile oil groups for set 1. The set 2 rats were divided into six groups (n = 7/group): rats received a regular diet or RD (C), RD with 1 ml (CCO1) and 3 ml (CCO3) of the oil per kg body weight, rats fed a high-fat diet or HFD (H), HFD with 1 ml (HCO1) and 3 ml (HCO3) of the oil per kg body weight. The results showed that the crocodile oil extracted using wet cold pressing showed no bottom clouded sediment with more transparent lightyellow color compared to the other oil samples. Moreover, it was shown that the crocodile oil treated rats had significantly lower food intake (p < 0.05) than the RD fed groups. The calculated total area of fat droplets in the liver tissues of the crocodile oil treated groups was significantly lower (p < 0.05) compared with the HFD and palm oil fed groups. Also, the increased phosphorylation of STAT3 accompanied by hunger suppression with the attenuation of NPY expression was higher in the crocodile oil treated groups. The results additionally revealed that HFD significantly increased the activity and relative gene expression of CYP1A2 in the H group (p < 0.05), whereas 3 ml per kg body weight of crocodile oil normalized the enzyme activities, which might be mediated by the omega-3 fatty acids found in the oil, compared to the C group. Also, the relative gene expression of GSTA1 was significantly decreased (p < 0.05) in the HCO1 and HCO3 groups, which might be attributed to the lower lipid peroxidation occurred in the liver tissues, compared to the H group. Therefore, it could be suggested that the modified wet cold pressing method could be adapted to further food and dietary supplement processing industries. Moreover, the oil lowered hunger expression and hepatic fat accumulation due to higher promotion of leptin sensitivity compared to HFD and palm oil. Also, it could be suggested that crocodile oil could help in liver detoxification through the CYP1A2 even when offered with HFD. Further studies investigating crocodile oil consumption in other animals and humans should be performed for further alternative uses in medical therapy.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Phitsanu Tulayakul

Role: Thesis Advisor

Name: Wirasak Fungfuang

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-27

Issued: 2025-07-27

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2565/pitchaya-san-all.pdf

CallNumber: TP676 .P58

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Bio-Veterinary Sciences

Grantor: Kasetsart University

©copyrights Kasetsart University

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