Cardioprotective effects of germinated brown rice (GBR) on H9c2 cardiomyocyte and porcine animal model

Name: Kanokwan Demeekul

keyword: Germinated brown rice.

LCSH: Kasetsart University -- Dissertations. Ph.D. (Bio-Veterinary Sciences) 2021

Classification :.LCCS: QP114.C44

LCSH: Kasetsart University. -- Faculty of Veterinary Medicine -- Dissertations

LCSH: Heart cells

LCSH: Coronary heart disease

LCSH: Cardiovascular system -- Diseases

LCSH: Cardiopulmonary bypass

LCSH: Brown rice -- Utilization

Abstract: Ischemic/reperfusion (I/R) injury is the fundamental mechanism during ischemic heart disease (IHD). The key modulator of I/R injury is the dysregulation of mitochondria function. Germinated brown rice (GBR) has been recognized as a bio-functional diet. However, the effect of GBR that mediated cardioprotective properties, focusing on mitochondrial function and myocardial protection remains unexplored. Thus, this study aimed to investigate the cardioprotective effects of GBR against simulated I/R injury and open-heart surgery under cardiopulmonary bypass (CPB) in a porcine animal model. H9c2 cardiomyocytes were incubated with GBR and/or simulated I/R injury. Cell viability and cell apoptosis were assessed by 7-AAD staining and AnnexinV/PI staining, respectively. The mitochondrial membrane potential was determined by JC-1 staining and mitochondrial respiration was represented by oxygen consumption rate (OCR). Primary porcine cardiomyocytes were isolated and experimented with cell viability against simulated I/R injury. In the porcine animal model of open-heart surgery under CPB, six pigs were randomly assigned to two groups, receiving St. Thomas cardioplegic solution and St. Thomas cardioplegic solution plus GBR. During surgical procedure, hemodynamic variables, arterial blood gas characteristics, and parameters of CPB were recorded until the end of the procedure. The results demonstrated that the pretreatment of GBR significantly reduced the percentage of cell death and total cell apoptosis in H9c2 stimulated with I/R. Furthermore, GBR remarkably stabilized mitochondrial membrane potential and improved the impairment of mitochondrial respiration in simulated I/R-induced H9c2 injury. Besides, GBR co-incubation with cardioplegic solution essentially resulted in the improvement of cell viability in isolated primary porcine cardiomyocytes against simulated I/R induction. Together with the findings from open-heart surgery, mean arterial pressure and heart rate were constantly stable in the GBR group. Consistently, all parameters from arterial blood gas showed better outcomes in animals receiving GBR, however, there were not statistically significant differences between groups, except hepatic enzymes. Thus, this is the first study to establish the effective cardioprotection of GBR. The underlying therapeutic activities of GBR are possibly associated with its bioactive components. However, the critical underlying mechanism on the cardioprotective properties of GBR requires further investigation.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Soontaree Petchdee

Role: Thesis Advisor

Created: 2021

Modified: 2025-07-26

Issued: 2025-07-26

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/kanokwan-dem-all.pdf

CallNumber: QP114.C44 .K36

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Bio-Veterinary Sciences

Grantor: Kasetsart University

©copyrights Kasetsart University

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