Molecular and serological detection of bartonella spp. in companion animals, livestock, and wildlife

Name: Phirabhat Saengsawang

LCSH: Kasetsart University -- Dissertations. Ph.D. (Agricultural Biotechnology) 2021

Classification :.LCCS: QR353.5.A5

LCSH: Kasetsart University. -- Interdisciplinary Graduate Program -- Dissertations

LCSH: Bartonella

LCSH: Bartonella infections in animals

LCSH: Gram-negative bacteria

Abstract: Bartonella spp. are Gram-negative and facultative intracellular bacteria causing several zoonotic and human diseases. Bartonella spp. is transmitted via various types of blood-sucking arthropods especially fleas. Additionally, ticks are suspected as a potential vector of Bartonella spp. Cats are confirmed as the natural hosts of these pathogens, however, dogs, humans, and other mammals are defined as accidental hosts. In the present, bartonellosis is pushed as an important disease for One-Health integration with the molecular and cellular investigation. Interestingly, there are few studies of Bartonella spp. in wild animals. The objectives of this study were to detect Bartonella spp. and to evaluate antibodies against Bartonella spp. in cats, dogs, cattle, goats, and elephants in Thailand. Whole blood and serum were collected from companion animals, livestock, and wildlife. Conventional polymerase chain reaction (cPCR) and bacterial culture were applied to detect bacteria in the host’s bloodstream meanwhile indirect immunofluorescent antibody test (IFAT) was performed to detect antibodies against Bartonella spp. For ectoparasites, all specimens were detected Bartonella spp. using cPCR. The prevalence of Bartonella spp. in cat fleas, dog ticks, and cats was 9.57%, 2.50%, and 2.53% respectively. Nevertheless, there was no evidence of Bartonella DNA in dogs, cattle, goats, and elephants. Bartonella henselae and B. clarridgeiae were detected in the blood of ectoparasite-exposed cats and cat fleas. Moreover, B. koehlerae and B. phoceensis were detected in brown dog ticks. For seroprevalence, antibodies against Bartonella spp. (≥1:64) were 13.16% and 22.51% in companion animals and livestock, respectively. Finally, the correlation between cPCR and IFAT of Bartonella spp. detection was no agreement (kappa = -0.04). Evidence of Bartonella spp. predominatly found in cats, cat fleas, and dog ticks. Furthermore, cats, dogs, and cattle were found antibodies against Bartonella spp. Besides, people who interect with these animals should aware the infection and ectoparasite control programs are needed to prevent the transmission of Bartonella spp.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Tawin Inpankaew

Role: Thesis Advisor

Name: Gunn Kaewmongkol

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-26

Issued: 2025-07-26

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/phirabhat-sae-all.pdf

CallNumber: QR353.5.A5 .P57

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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