Chemical lure development as attractants for diurnal mosquito, aedes aegypti and nocturnal mosquito, culex quinquefasciatus surveillance tools

Name: Kim, Daeyun

LCSH: Kasetsart University -- Dissertations. Ph.D. (Entomology) 2021

Classification :.LCCS: QL536

LCSH: Kasetsart University. -- Department of Entomology -- Dissertations

LCSH: Culex quinquefasciatus

LCSH: Aedes aegypti

LCSH: Mosquitoes

LCSH: Olfactometry

LCSH: Dengue

LCSH: Vector control

Abstract: The aim of this study was to develop a methodology consisting of a laboratory apparatus and procedure for developing mosquito lures to improve field surveillance of the medically important mosquito vectors Aedes aegypti (L.) and Culex quinquefasciatus Say. This involved use of a diffusion assay based on a high-throughput screening system (HITSS) olfactometer, a semi-field screened house (SFSH), and metrics to evaluate the attraction of different candidate lures. The HITSS assay initially was optimized to account for dose-response variability using the commercially available BG-LureTM (Biogents). Subsequently, four lure candidates consisting of newly formulated L-lactic acid-based chemical mixtures developed at Kasetsart University (referred to as KU-lure #1, KU-lure #4, KU-lure #5, and KU-lure #6) along with the single chemicals isoamyl alcohol (KU-lure #2) and octenol (KU-lure #3) were tested. The assays found the BG-lure (10g pack) strongly repelled Ae. aegypti and Cx. quinquefasciatus exhibited negative percent attractions of -96.3% ± 11.1% and -50.2% ± 34.7%, respectively. However, the optimized dose of 0.05g of the BG-lure showed positive values for both species of 53.5% ± 31.8% and 68.3% ± 25.4%, respectively. The four KU-lure candidates and those with the two pure components were screened using a discriminating doses of 0.01 g. Species-specific preferences were observed such that Ae. aegypti preferred KU-lure #1 (29.7% ± 31.4%) while KU-lure #6 was preferred by Cx. quinquefasciatus (37.2% ± 47.6%). Interestingly, Cx. quinquefascaitus responded significantly more (76.0% ± 31.4%) than Ae. aegypti (12.2% ± 31.4%) to 1.0 g of dry ice in the 2.75L of HITSS (p = 0.002). This phenomenon was repeated in the 140 m3 volume of semi-field screen house (SFSH) assay (75.1% ± 16.9% vs 42.2% ± 14.2%, p < 0.05) using 250 ml/minutes of carbon dioxide flow rate from a tank. In addition, the SFSH assay demonstrated the accuracy of the HITSS assay as the KUlures #1 and #6 treated BG SentinelTM traps resulted significantly increased percent attraction for Ae. aegypti (29.5% ± 14.3%) and Cx. quinquefasciatus (33.3% ± 10.7%) compared to untreated traps (0.8% ± 11.2% and -5.0% ± 29.4%, respectively). Overall, the study demonstrated the HITSS assay system is suitable for evaluating the potential lure candidates, thus contributing to the improvement of surveillance tools for both diurnal and nocturnal mosquitoes.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Theeraphap Chareonviriyaphap

Role: Thesis Advisor

Name: Bruce Alexander Wilcox

Role: Thesis CO-Advisor

Name: Theerachart Leepasert

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-26

Issued: 2025-07-26

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2563/daeyun-kim-all.pdf

CallNumber: QL536 .K56

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Entomology

Grantor: Kasetsart University

©copyrights Kasetsart University

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