Effect of alpha-mangostin and alpha-mangostin-loaded nanostructured lipid carriers on mice models of cerebral ischemia-reperfusion and Parkinsons disease

Name: Romgase Sakamola

LCSH: Kasetsart University -- Dissertations. Ph.D. (Zoology) 2021

Classification :.LCCS: RB144.5

LCSH: Kasetsart University. -- Department of Zoology -- Dissertations

LCSH: Reperfusion injury

LCSH: Cerebrovascular disease

LCSH: Cerebral ischemia

LCSH: Parkinson's disease

LCSH: Ischemia

LCSH: Nanoparticles

LCSH: Lipids

Abstract: Natural compounds have been popularly used as neuroprotective and delaying agents in neurodegenerative diseases. Alpha-mangostin (αM) is a natural xanthone derivative from mangosteen which possesses antioxidative and protective properties against brain disorders, but the in vivo application of αM is limited due to its low solubility and bioavailability. Nanostructured lipid carriers (NLC) are drug delivery systems that improve the limitations of natural compounds and enhance their efficacy. This study investigated the effect of αM and αM-NLC in mice models of ischemia-reperfusion (IR) injury and Parkinson’s disease (PD). In the IR model, forty-male ICR mice were divided into four groups: Sham, IR, IR with 25 mg/kg of αM (IR+αM), and IR with 25 mg/kg of αM-NLC (IR+αM-NLC). After 6 days of oral administrations, IR was induced using 30 min bilateral common carotid artery occlusion followed by 45 min of reperfusion. Brain infarction volume, brain’s oxidative status, hippocampus and corpus callosum (CC) histopathology were evaluated. In the PD model, forty-male ICR mice were divided into 4 groups: Normal, PD, PD+αM, and PD+αM-NLC. Vehicle or treatment was orally administered 1 hour before rotenone-induced PD injection (2.5mg/kg/48 hours) for 4 consecutive weeks. Weekly motor coordination, muscle strength and anxiety were evaluated using rotarod, hanging wire, and elevated plus maze (EPM) tests, respectively. After finishing of behavioral tests, brain’s oxidative status, neuronal population in motor cortex, striatum, and substantia nigra were evaluated. Tyrosine hydroxylase (TH) immunostaining also used in striatum and substantia nigra for dopaminergic neuronal observation. Results found that αM exhibited protective effect against hippocampal CA1 degeneration, CC white matter damage, and MDA level in IR mice. While αM-NLC significantly decreased brain infarction, hippocampal CA1, and CA3 degeneration, CC white matter damage and MDA. αM-NLC also increased SOD activity in mice with IR. In PD mice, αM improved motor deficits, protected against neuronal degeneration in substantia nigra, and increased TH in both striatum and substantia nigra. While αM-NLC improved motor deficits and anxiety-like behavior, decreased brain oxidation and neuronal degeneration in motor cortex, striatum, and substantia nigra. αM-NLC also increased TH in striatum and substantia nigra in PD mice. Therefore, αM still exerts a neuroprotective against IR and PD in mice and NLC emphasizes the efficacy of αM.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Wachiryah Thong-Asa

Role: Thesis Advisor

Name: Wirasak Fungfuang

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-25

Issued: 2025-07-25

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/romgase-sak-all.pdf

CallNumber: RB144.5 .R66

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degraee

Descipline: Zoology

Grantor: Kasetsart University

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