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Visakha Chunhakorn. Development of monoliths and microchannels for protein separation and Polyaromatic Hydrocarbon extraction. Doctoral Degraee(Physics). Kasetsart University. Office of the University Library. : Kasetsart University, 2018.
Development of monoliths and microchannels for protein separation and Polyaromatic Hydrocarbon extraction
Name: Visakha Chunhakorn
Classification :.LCCS: QD431
LCSH:
Proteins
-- Separation
LCSH:
Microfluidics
LCSH:
Microfabrication
LCSH:
Placental extracts
Abstract:
The development of monolithic materials for use in analytical chemistry using with microfluidic chips has been investigated. The first part of the work, the suitability of materials, namely polydimethylsiloxane (PDMS), glass, and polytetrafluoroethylene (PTFE), for analytical microfluidic chip was studied. The octyl (C8-) and octadecyl (C18-) silica monolith was synthesised by an in-situ sol gel reaction in the channels in both PDMS and glass microchip. The PTFE microfluidic chips were only successfully fabricated as evidenced by water flow through the channel. Characterisation of the material in the channel revealed the synthesised C18 monolith had small skeleton with macropores of a few micrometres. The second investigation dealt with the fabrication of C18- silica based monolith into a fused silica capillary that was subsequently used as column for liquid chromatography combined with mass spectrometer (nanoLC-MS/MS) for a study of protein identification in porcine placenta extract. The home-made column can be connected and operated under the same conditions as a commercial column. However, the efficiency of home-made column was inadequate compared to the standard commercial column for good separation. In the third study, polystyrene-divinyl benzene (PS-DVB) monolithic bead were developed for extraction of polycyclic aromatic hydrocarbons (PAHs) in water samples, and the analysis was achieved by gas chromatography with mass spectroscopic detection (GC-MS). A procedure for analysis of 16 PAHs in different water samples was investigated. The calibration curves showed high levels of linearity of r2 >0.995 for all PAHs in the range of 230,000 ng mL-1. The limits of detection (LODs) ranged between 0.01-0.47 ng mL-1 . % recoveries of PAHs in spiked water samples at 500 ng mL-1 were 72-109%, while for the analysis of 20 ng mL-1 of target analytes added to actual water samples spanned 69-114%. Both intraday and interday respectabilities in terms of %RSD for 16 PAH-spiked standard solutions were below 10%. The performance of the method for determining of PAHs in environmental and drinking water samples was evaluated, showing satisfactory result. The simplicity of the method makes it eminently well suited for practical use.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Orapin Chienthavorn
Role:
Thesis Advisor
Name: Harry James Whitlow
Role:
Thesis CO-Advisor
Name: Chomdao Sinthuvanich
Role:
Thesis CO-Advisor
Created:
2018
Modified:
2025-07-25
Issued:
2025-07-25
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2561/visakha-chu-all.pdf
CallNumber:
QD431 .V57
eng
DegreeName: Doctor of Philosophy
Level: Doctoral Degraee
Descipline: Physics
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.024873 วินาที
Visakha Chunhakorn
| Title | Contributor | Type |
|---|---|---|
| Monolithic materials for microfluidic system
มหาวิทยาลัยเกษตรศาสตร์ Visakha Chunhakorn | Orapin Chienthavorn | วิทยานิพนธ์/Thesis |
| Development of monoliths and microchannels for protein separation and Polyaromatic Hydrocarbon extraction
มหาวิทยาลัยเกษตรศาสตร์ Visakha Chunhakorn | Orapin Chienthavorn | วิทยานิพนธ์/Thesis |
Orapin Chienthavorn
Harry James Whitlow
| Title | Creator | Type and Date Create |
|---|
Chomdao Sinthuvanich