Determination of histamine in gryllus bimaculatus and acheta domesticus after post-harvest processing by HPLC-UV method

Name: Chayanon Chumjai

LCSH: Kasetsart University -- Theses. M.S. (Biochemistry) 2023

Classification :.LCCS: QP801.H5

LCSH: Kasetsart University. -- Department of Biochemistry -- Theses

LCSH: Histamine

LCSH: Edible insects

LCSH: Acheta

LCSH: Crickets

Abstract: Edible cricket has attracted the attention of consumers and the food industry as an alternative high-protein food source. However, in view of safety, the information on biogenic amine contents, particularly histamine, was still lacking. In this work, our study aims to quantify the level of histamine in edible crickets using the HPLC-UV method and to identify the factors that contribute to the histamine levels in edible crickets for post-harvest management purposes. Here, the histamine levels in two species of edible crickets, Acheta domesticus and Gryllus bimaculatus were measured. The linearity, limit of detection, limit of quantitation, precision, and recovery were validated. Our findings revealed that histamine levels in crickets increased when stored at room temperature. In contrast, storage of raw or cooked edible crickets at -20 °C or 4°C significantly delayed histamine accumulation regardless of cricket species. Furthermore, we found that over 95% of farmed crickets from forty-four farms in Thailand contained histamine levels of less than 50 mg/kg. The duration of boiling impacted the accumulation of histamine in cooked farmed crickets, while no significant association was detected between the histamine level in cricket and the farming practices, seasoning, and transportation duration.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chomdao Sinthuvanich

Role: Thesis Advisor

Name: Chama Inson

Role: Thesis CO-Advisor

Name: Namfon Tongtavee

Role: Thesis CO-Advisor

Created: 2023

Modified: 2025-07-24

Issued: 2025-07-24

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2566/chayanon-chu-all.pdf

CallNumber: QP801.H5 .C43

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Kasetsart University

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