แจ้งเอกสารไม่ครบถ้วน, ไม่ตรงกับชื่อเรื่อง หรือมีข้อผิดพลาดเกี่ยวกับเอกสาร ติดต่อที่นี่ ==>
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
Supapitch Uten. The study of magnetic Graphene oxide for microRNA isolation. Master's Degree(Chemistry). Kasetsart University. Office of the University Library. : Kasetsart University, 2021.
The study of magnetic Graphene oxide for microRNA isolation
Name: Supapitch Uten
Classification :.LCCS: TA455.G65
LCSH:
Graphene oxide
LCSH:
Graphene
-- Magnetic properties
LCSH:
Ferric oxide
LCSH:
MicroRNA
Abstract:
Recently, microRNAs (miRNAs) have emerged as one of the novels and promising biomarkers in both human and veterinary clinical diagnostics because of their abundance, stability in blood and biofluids, and their significant roles in various biological processes. The isolation of miRNAs is an initially important step for further utilizations of miRNAs. However, fluid-based methods such as phenol-chloroform, silica membrane-based adsorption extraction kits are complicated, time-consuming, expensive, and toxic. Therefore, the development of an easy, rapid, biocompatible, and cost-effective miRNAs isolation is studied herein. Graphene oxide (GO) nanomaterial, a hydrophilic planar sheet with high surface area, possessed with a capacity to immobilize nucleotides via π-π stacking with enhancing stability is chosen. The chemical functionalization of GO with amine-modified Fe3O4 nanoparticles has been synthesized to demonstrate the miRNAs extraction associated with the magnetic separation. The synthesized Fe3O4@GO is characterized by the combination of X-ray Diffraction Spectroscopy (XRD), Fourier-Transform Infrared Spectroscopy (FTIR), Raman Spectroscopy, Atomic Force Microscopy (AFM), Transmission Electron Microscopy (TEM), UVVisible spectrophotometer, Thermal Gravimetric Analysis (TGA), Vibrating Sample Magnetometer (VSM) and Dynamic Light Scattering (DLS) techniques. It showed that 9 nm size-controlled and aminated Fe3O4 was conjugated with GO through chemical covalent bonding at the edge of GO. The percentages of Fe3O4 nanoparticles loading onto GO are also studied to investigate the separation process. The recovery of fluorescence intensity confirmed the ability to use Magnetic GO developed for efficient and high-purity miRNA isolation.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Patraporn Luksirikul
Role:
Thesis Advisor
Created:
2021
Modified:
2025-07-24
Issued:
2025-07-24
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2564/supapitch-ute-all.pdf
CallNumber:
TA455.G65 .S87
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Chemistry
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
-0.921554 วินาที
Supapitch Uten
| Title | Contributor | Type |
|---|---|---|
| The study of magnetic Graphene oxide for microRNA isolation
มหาวิทยาลัยเกษตรศาสตร์ Supapitch Uten | Patraporn Luksirikul | วิทยานิพนธ์/Thesis |
Patraporn Luksirikul
| Title | Creator | Type and Date Create |
|---|---|---|
| Direct synthesis of hierarchical ferrierite nanosheet assemblies via the organosilane template approach and their catalytic behaviors
มหาวิทยาลัยเกษตรศาสตร์ Pipat Khongpracha ;Patraporn Luksirikul ;Waraporn Parasuk | Phatsawit Wuamprakhon | วิทยานิพนธ์/Thesis |
| One-pot synthesis of hierarchical ZSM-5 via an organosilane surfactant approach for bio-oil upgrading application
มหาวิทยาลัยเกษตรศาสตร์ Chompunuch Warakulwit ;Patraporn Luksirikul ;Waraporn Parasuk | Kamonlatth Rodponthukwaji | วิทยานิพนธ์/Thesis |
| The study of magnetic Graphene oxide for microRNA isolation
มหาวิทยาลัยเกษตรศาสตร์ Patraporn Luksirikul | Supapitch Uten | วิทยานิพนธ์/Thesis |