Molecular characterization of Antimicrobial resistance Klebsiella spp. isolated from slaughtered Pigs in Thailand

Name: Nattamol Phetburom

LCSH: Kasetsart University -- Theses. M.P.H. 2020

Classification :.LCCS: QR82.E6

LCSH: Kasetsart University. -- Department of Public Health Administration -- Theses

LCSH: Drug resistance in microorganisms

LCSH: Klebsiella

LCSH: Enterobacteriaceae

LCSH: Slaughtering and slaughter-houses -- Thailand

Abstract: The emergence of antimicrobial resistance in Klebsiella spp., including resistance on 3 rd generation cephalosporins, carbapenem, and colistin in human, animals, and environment is public health concern. The aim of this study was to determine the phenotypic and genotypic characteristics of antimicrobial resistance in Klebsiella spp. isolated from slaughtered pigs in Thailand. A total of 384 Klebsiella spp. was characterized antimicrobial resistance genes by polymerase chain reaction (PCR), antimicrobial susceptibility, and mobile colistin resistance gene transferable by conjugation assay. The results showed 246 K. pneumoniae (64.06%), 72 K. oxytoca (18.75%), 25 K. quasipneumoniae (6.51%), 23 K. varricol a (5.98%), and 18 K. aerogenes (4.68%). Mobile colistin resistant gene (mcr) was detected in K. pneumoniae complex (KpnC: K. pneumoniae, K. variicola, and K. quasipneumoniae) (20/384; 5.20%) that composed of mcr-8 (9/20; 45%), mcr-7 (7/20; 35%), mcr-7+mcr-8 (3/20; 15%), and mcr-1+mcr-7 (1/20; 5%). Transfer of colistin resistance genes was determined in 20 mcr-harboring of KpnC isolates. E. coli, 6 KpnC donors (30%) could transfer mcr-1 (5%) and mcr-8 (25%) to recipient. All transconjugants exhibited minimal inhibitory concentration (MIC) of colistin in the range of 4-8 µg/ml. In addition, 384 isolates of Klebsiella spp. were detected 84.37% β lactamases genes, 63% plasmid-mediated quinolone resistance genes (PMQR), and 2.1% carbapenem-resistant genes. This finding indicates that the antimicrobial resistance of Klebsiella spp. from slaughtered pigs is one of the reservoirs for resistance dissemination Therefore the use of antimicrobial in animal husbandry and agriculture should be avoided to prevent and control of antimicrobial resistance bacteria spreading.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Parichart Boueroy

Role: Thesis Advisor

Name: Anusak Kerdsin

Role: Thesis CO-Advisor

Created: 2020

Modified: 2025-07-21

Issued: 2025-07-21

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2563/nattamol-phe-all.pdf

CallNumber: QR82.E6 .N38

eng

Spatial: Thailand

DegreeName: Master of Public Health

Level: Master's Degree

Descipline: Public Health

Grantor: Kasetsart University

©copyrights Kasetsart University

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