Transcriptome analysis in different tissues, characterization and functional analyses of potent pattern recognition receptors (PRRs) in Adult Pacific oyster (crassostrea gigas) Infected with virulence vibrio parahaemolyticus

Name: Petcharat Namwong

LCSH: Kasetsart University -- Theses. M.S. (Fishery Science and Technology) 2022

Classification :.LCCS: QL430.7.O9

LCSH: Kasetsart University. -- Faculty of Fisheries -- Theses

LCSH: Pacific oyster

LCSH: Pacific oyster -- Genetics

LCSH: Vibrio parahaemolyticus

LCSH: Immune recognition

Abstract: The Pacific oyster (Crassostrea gigas) is an economically important marine bivalve species annually monitored for risk assignment of Vibrio parahaemolyticus contamination, which lead to the outbreak of gastroenteritis infection and public health concern in humans. To prevent a rising outbreak of human and infectious diseases in the oyster aquaculture industry, research has been developed in the post-harvest process (PHP) and genetic improvement. However, understanding host-microbial interaction based on pattern recognition receptors (PPRs) in the digestive gland and mantle tissues is still poor. Then, transcriptomic analysis was adopted in this study to discover the interaction of global gene expression in terms of immune and PRRs response. In this study, 18 expressed gene libraries were constructed in the digestive gland and mantle tissues of Pacific oysters infected with V. parahaemolyticus. As volcano plotting and GO and KEGG enrichment analysis based on differential genes expressions (DEGs), the upregulated genes expression data sets of digestive gland tissues indicated significantly expressed on immune mechanisms about genes response on Gramnegative bacterial adhesion against epithelium host cells and phagocytosis. In contrast, mantle tissue demonstrated significant oxidative stress and peroxidase reaction response. In contrast, down-regulated genes expressed have similarly provided gene-related with the movement of cells based on the cytoskeleton in all tissues. Focused on genes involving immune and Gram-negative bacterial infection, CgCTL, Cgperlucin-like, CgCL-11, CgMCR1, CgGal-9, and CgLBP demonstrated significantly up-regulated expression. Furthermore, CgPGRP-SC2X2, CgβGBP, CgC1q, CgToll-like 4, and CgCASP-3, were also significantly expressed postinfection. For better understanding, CgCTL, Cgperlucin-like, CgLBP, CgPGRPSC2X2, CgβGBP, CgMCR1, and CgGal-9 were selected as candidates in the protein binding function analysis via indirect immunofluorescent assay (IFA). The result revealed that rCgGal-9 and rCgMCR1 contained carbohydrate-binding domains (CBDs), demonstrated strong binding and agglutination functions with the outer layer of cell membrane with V. parahaemolyticus, clinical isolation and ATCC 17802 strains, more than rCgβGBP and rCgPGRP-SC2X2. In summary, this study points out the lists of genes related to immune mechanism and characteristics of agglutination and binding function of PRRs genes on the V. parahaemolyticus cell wall for improved information to further studies of the alternative strategies in post-harvest process and prevention.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Prapansak Srisapoome

Role: Thesis Advisor

Name: Qing Kong

Role: Thesis CO-Advisor

Created: 2022

Modified: 2025-07-20

Issued: 2025-07-20

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2565/petcharat-nam-all.pdf

CallNumber: QL430.7.O9 .P48

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Fishery Science and Technology

Grantor: Kasetsart University

©copyrights Kasetsart University

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