Transformation of oil palm baby boom (BBM) gene into rice calli using agrobacterium and carbon nanotube delivery methods

Name: Paphawarin Pinyokham

LCSH: Kasetsart University -- Theses. M.S. (Genetics) 2021

Classification :.LCCS: QK495.P17

LCSH: Kasetsart University. -- Department of Genetics -- Theses

LCSH: Oil Palm -- Genetics

LCSH: Agrobacterium

LCSH: Carbon nanotubes

LCSH: Plant genetics

LCSH: Genetic transformation

Abstract: Oil palm is an important economic oil crop. A cross between two oil palm trees will result in a segregation of progeny. Tissue culture is the only method to propagate the true-to type oil palm tree. However, tissue culture of oil palm, especially the somatic embryogenesis step, takes a long duration time. BABY BOOM (BBM) gene, encoding a transcription factor, is a key gene that regulates plant cell totipotency undergoing somatic embryogenesis. In this study, we aimed to permanently and transiently express the oil palm EgBBM gene in rice callus using two different methods: Agrobacterium co-cultivation and carbon nanotube transformation. The transformation protocol using single-walled carbon nanotube (SWCNT) was optimized. This protocol used plastic medical syringe to create the physical pressure for the delivery of the DNA plasmid into plant cell. 35S:mCherry:pCXSN plasmid vector was successfully delivered into rice callus. The strong red fluorescence signal from mCherry protein was observed. The quantitative analysis of mCherry mean intensity revealed that the mCherry signal intensity of carbon nanotube + plasmid treatment was highly significantly different from the control treatments both at 3 days- and decreased at 6 days post-transformation. This transient transformation protocol is rapid, easy to perform and highly efficient to transport plasmid DNA into plant callus, which can be applied for gene functional analysis and protein production.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chatchawan Jantasuriyarat

Role: Thesis Advisor

Name: Supachai Vuttipongchaikij

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-20

Issued: 2025-07-20

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/paphawarin-pin-all.pdf

CallNumber: QK495.P17 .P37

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Genetics

Grantor: Kasetsart University

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