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Thanathip Sutthiphai. Genetic variation of Avirulence genes (AVR-Pi9, AVR-Pik, AVR-Pita) and genetic diversity of Rice blast fungus, Pyricularia oryzae in Thailand. Master's Degree(Genetics). Kasetsart University. Office of the University Library. : Kasetsart University, 2021.
Genetic variation of Avirulence genes (AVR-Pi9, AVR-Pik, AVR-Pita) and genetic diversity of Rice blast fungus, Pyricularia oryzae in Thailand
Name: Thanathip Sutthiphai
Classification :.LCCS: QK495.G74
LCSH:
Pyricularia oryzae
LCSH:
Rice blast disease
LCSH:
Virulence (Microbiology
Abstract:
Genetic variation of rice blast population was investigated based on the nucleotide sequence of three avirulent genes, AVR-Pi9, AVR-Pik and AVR-Pita1. Sixty rice blast isolates were collected from rice-growing areas around Thailand. The gene-specific primers were used to amplify these AVR genes. The results showed that AVR-Pi9, AVR-Pik and AVR-Pita1 were detected in all, 57 and 23 isolates, respectively. Based on the AVR-Pi9 sequences, we identified one rice blast isolate containing an amino acid change from glutamic acid to aspartic acid. Moreover, we found two rice blast isolates with identical sequence to the rice blast strain originating in Japan. This finding indicates that there is a movement of the rice blast isolate from Japan to Thailand. Three AVR-Pik alleles were found, including AVR-PikA (3.51%), AVR-PikD (71.93%), and isolates with 2 copies of AVR-PikD and AVR-PikF (24.56%). AVR-PikA and AVR-PikF are virulent to Thai rice variety, Jao Hom Nin. Six haplotypes of AVR-Pita1 were identified with one deletion and twelve amino acid substitutions. The results from this study revealed that different AVR genes in Thai rice blast populations have different levels of genetic variation. The AVR-Pi9 and AVR-Pik genes have a relatively low genetic diversity, while the AVR-Pita1 gene has a high genetic diversity. The AVRPi9 was not under selection pressure, while AVR-Pita1 was under purifying selection pressure. The geographical location has influenced the distribution of the AVR-Pita1 gene genetic variation. The information obtained from this study is valuable for the development of rice blast resistance breeding strategies for Thailand in the future.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Chatuporn Kuleung
Role:
Thesis Advisor
Role:
Thesis CO-Advisor
Created:
2021
Modified:
2025-07-18
Issued:
2025-07-18
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2564/thanathip-sut-all.pdf
CallNumber:
QK495.G74 .T46
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Genetics
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
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0.025282 วินาที
Thanathip Sutthiphai
| Title | Contributor | Type |
|---|---|---|
| Genetic variation of Avirulence genes (AVR-Pi9, AVR-Pik, AVR-Pita) and genetic diversity of Rice blast fungus, Pyricularia oryzae in Thailand
มหาวิทยาลัยเกษตรศาสตร์ Thanathip Sutthiphai | Chatuporn Kuleung | วิทยานิพนธ์/Thesis |
Chatuporn Kuleung
| Title | Creator | Type and Date Create |
|---|---|---|
| Population genetic analysis of magnaporthe oryzae in sub-Saharan Africa using SNP calling from whole genome sequencing and de novo assembly of magnaporthe oryzae from Pacbio and Illumina Sequencing
มหาวิทยาลัยเกษตรศาสตร์ Chatuporn Kuleung | Worrawit Suktrakul | วิทยานิพนธ์/Thesis |
| Genetic variation of Avirulence genes (AVR-Pi9, AVR-Pik, AVR-Pita) and genetic diversity of Rice blast fungus, Pyricularia oryzae in Thailand
มหาวิทยาลัยเกษตรศาสตร์ Chatuporn Kuleung | Thanathip Sutthiphai | วิทยานิพนธ์/Thesis |
Chatchawan Jantasuriyarat