Application of effectively probiotic Bacillus spp for enhancement of growth performance, immune responses, and disease resistance in Asian Seabass (Lates calcarifer, Bloch)

Name: Kanaka Malleswara Rao Vissamsetty, Veera Venkata

LCSH: Kasetsart University -- Theses. M.S. (Fishery Science and Technology) 2021

Classification :.LCCS: SH167.S33

LCSH: Kasetsart University. -- Faculty of Fisheries -- Theses

LCSH: Sea basses -- Feeding and feeds

LCSH: Sea basses -- Growth

LCSH: Giant perch -- Feeding and feeds

LCSH: Giant perch -- Growth

LCSH: Bacillus (Bacteria)

LCSH: Probiotics

Abstract: Asian seabass (Lates calcarifer, Bloch) is an important commercial and subsistence fish in Southeast Asia and Australia. Aquaculture production of Asian seabass is a rapidly growing enterprise with a rapidly expanding market as a popular seafood item in many European and North American countries. To date, diseases caused by bacteria greatly damage the culture of Asian seabass worldwide. Streptococcus agalactiae is the causative agent of Streptococcosis disease. The Pathogenic bacteria's zones are inhibited by this three Bacillus spp. at 0.90± 0.100, 2.40± 0.200, and 2.50± 0.265 mm, respectively. B. subtilis possessed some unique qualities. B. amyloliquefaciens and B. pumilus were shown to be the most effective at controlling Streptococcus agalactiae. After 120 hours of incubation, the inclusion of Bacillus spp. B4, B6, and B12 resulted in significant inhibition of Streptococcus agalactiae serotype-Ia growth. Whereas there was minimal difference detected in Bacillus spp. at the end of the co-culture experiment. As a result, B4, B6, and B12 may be recommended for use as mono or combination fish probiotics, Application effects of probiotic supplemented feed by various probiotic patterns on growth, immune responses, and disease resistance to Streptococcus agalactiae in 12-gram Asian seabass for 4 weeks including; 1) Control group without probiotic supplemented feed (C) 2) Feed supplemented with Bacillus pumilus (B4) 3) Feed supplemented with Bacillus subtilis (B12) 4) Feed supplemented with Bacillus pumilus + Bacillus subtilis (B4+B12) 5) Feed supplemented with Bacillus pumilus + Bacillus subtilis + Vitamin C(B4+B12+VC) were investigated. The results revealed that fish groups obtained feed supplemented with B4 and B4+B12 showed average daily gain (ADG) and specific growth rate (SGR) at weeks 1 and 3 significantly higher than other groups (P<0.05). Immune response analyses indicated that the fish group that consumed feed supplemented with B12 significantly expressed nitro blue tetrazolium (NBT) dye reaction at week 1 higher than other groups (P<0.05). However at week 4, fish in all 5 groups were intraperitoneally injected with S. agalactiae for 10 days showed that fish that obtained B4 and B4+B12 in feed demonstrated significantly lower mortality than those of other groups (P<0.05) with 0.00±0.00 and 10.00±0.00, respectively. While fish in the control group, fish consumed B12 and fish consumed B4+B12+VC(1gms/Kg) showed accumulative of 60.00±20.00, 35.00±5.00, and 20.00±0.00, respectively.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Methee Kaewnern

Role: Thesis Advisor

Name: Narongrit Muangmai

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-18

Issued: 2025-07-18

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/veera-ven-all.pdf

CallNumber: SH167.S33 .K36

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Fishery Science and Technology

Grantor: Kasetsart University

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	veera-ven-all.pdf	1.8 MB

ใช้เวลา

0.028084 วินาที