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Naiyasit Yingkamhaeng. Synergistic effect of Xylanase and Laccase pretreatment on cellulose extraction obtained from energy cane. Doctoral Degraee(Biotechnology). Kasetsart University. Office of the University Library. : Kasetsart University, 2021.
Synergistic effect of Xylanase and Laccase pretreatment on cellulose extraction obtained from energy cane
Name: Naiyasit Yingkamhaeng
Classification :.LCCS: TP248.65.L54
LCSH:
Lignocellulose
LCSH:
Cellulose
LCSH:
Laccase
LCSH:
Xylanase
LCSH:
Biomass conversion
Abstract:
Currently, xylanase and laccase are used in several applications of biobleaching industry. However, combined operation of these two enzymes has limitations due to their different functional conditions. Therefore, this study aimed to elucidate the synergistic effect of xylanase and laccase conditions on bleaching of energy cane (EC). Temperature and pH profiles of xylanase and laccase activities were determined to use for enzymatic treatment condition. Results showed that a single pretreatment of 50 U/g xylanase and 100 U/g laccase significantly enhanced the whiteness index of pretreated fibers by 85%. Whiteness index increased to 95% when biobleaching synergistic xylanase/laccase treatment was applied. Synergistic xylanase/laccasetreated fibers showed 70% xylan removal, with enhanced crystallinity index at 59% compared to single xylanase and laccase-treated fibers. Synergistic xylanase/laccase treatment increased the specific surface area (2.8 m2 /g) and surface roughness (81.83 μm) of the fibers, indicating partial xylan hydrolysis and lignin depolymerization. NMR spectroscopy exhibited bond cleavage of lignin-xylan and lignin inter-unit linkages due to enzyme synergistic actions. Results increase the understanding of synergistic action mechanisms between xylanase and laccase to improve biobleaching efficiency and can be implemented to enhance biorefinery production of various lignocellulosic biomass.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Prakit Sukyai
Role:
Thesis Advisor
Name: Thidarat Nimchua
Role:
Thesis CO-Advisor
Created:
2021
Modified:
2025-07-12
Issued:
2025-07-12
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2564/naiyasit-yin-all.pdf
CallNumber:
TP248.65.L54 .N35
eng
DegreeName: Doctor of Philosophy
Level: Doctoral Degraee
Descipline: Biotechnology
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.026349 วินาที
Naiyasit Yingkamhaeng
| Title | Contributor | Type |
|---|---|---|
| Isolation and characterization of nanocellulose from mango and rambutan peets obtained by steam explosion combined with chemical treatments
มหาวิทยาลัยเกษตรศาสตร์ Naiyasit Yingkamhaeng | Prakit Sukyai | วิทยานิพนธ์/Thesis |
| Synergistic effect of Xylanase and Laccase pretreatment on cellulose extraction obtained from energy cane
มหาวิทยาลัยเกษตรศาสตร์ Naiyasit Yingkamhaeng | Prakit Sukyai | วิทยานิพนธ์/Thesis |
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