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Pongsak Lowmunkhong. Method development for determinatioin of chloramphenicol and semicarbazide in food samples. Doctoral Degraee(Chemistry). Kasetsart University. Office of the University Library. : Kasetsart University, 2020.
Method development for determinatioin of chloramphenicol and semicarbazide in food samples
Name: Pongsak Lowmunkhong
Classification :.LCCS: RM666.C5215
LCSH:
Chloramphenicol
LCSH:
Molecular imprinting
Abstract:
Chemical contaminants in food receive great attention due to their adverse effect on human health. The present work thus has developed two methods for food sample analysis. The first method involved confirmatory determination of chloramphenicol in honey using molecularly imprinted polymer (MIP) and LCMS/MS detection, and the second method dealed with determination of semicarbazide in shrimp by derivatization with 2-naphthaldehyde and detection with tandem LC-IPAD . In the first method, the MIP was prepared using a noncovalent and microwave heating-induced polymerization method at 80°C. 4-Vinylpyridine, a mixture of trimethylolpropane trimethacrylate and 1,4-divinylbenzene , 1,1- azobis(cyclohexane carbonitrile) and Pluronic P123 were used as a monomer, crosslinkers, an initiator and a surfactant, respectively, in a mixture of acetonitrile (MeCN) and water. The polymer was used as a solid phase extraction sorbent in the cleanup step of honey extracts which were separated on a Lichrospher®100 RP 8 column (5.0 μm, 125 × 4 mm) and detected in multireaction monitoring mode at m/z 320.9 → 151.8 and 320.9 → 256.8 transitions. Matrix matched calibration curve with d5-CAP as the internal standard was in the linear range of 0-2.0 µg/kg with a limit detection of 0.022 µg/kg and the mean recoveries of 91.9 -104.6 % with intraday and inter-day precisions less than or equal to 7.9 % and 9.3 %, respectively. Of ten samples tested, one sample contained chloramphenicol greater than 0.3 µg/kg. In the second method, released semicarbazide in shrimp was first derivatized with 2-naphthaldehyde and the extract was then cleaned up with liquid -liquid solvent extraction followed by separation on tandem LC column and detection with IPAD. The linear range was 0.0-3.0 µg/kg with a limit detection of 0.132 µg/kg and a precision of 13.51%. Of five sample tested, none was found to contain SEM greater than 1µg/kg.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Pakawadee Sutthivaiyakit
Role:
Thesis Advisor
Created:
2020
Modified:
2025-07-11
Issued:
2025-07-11
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2563/pongsak-low-all.pdf
CallNumber:
RM666.C5215 .P66
eng
DegreeName: Doctor of Philosophy
Level: Doctoral Degraee
Descipline: Chemistry
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.021287 วินาที
Pongsak Lowmunkhong
| Title | Contributor | Type |
|---|---|---|
| Studies of corrosion inhibitors for mild steel by delectrochemical techniques
มหาวิทยาลัยเกษตรศาสตร์ Pongsak Lowmunkhong | Pakawadee Sutthivaiyakit | วิทยานิพนธ์/Thesis |
| Paper-based ammonia gas sensor using zinc complex of Eriochrome Black T and digital image analysis
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Supa Sangdang;Pongsak Lowmunkhong;Pakawadee Sutthivaiyakit | บทความ/Article | |
| Simultaneous determination of p-coumaric acid and naringenin in honey using dispersive liquid-liquid microextraction and high performance liquid chromatography
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Korbkarn Khukitirat;Pongsak Lowmunkhong;Somyote Sutthivaiyakit;Pakawadee Sutthivaiyakit | บทความ/Article | |
| Method development for determinatioin of chloramphenicol and semicarbazide in food samples
มหาวิทยาลัยเกษตรศาสตร์ Pongsak Lowmunkhong | Pakawadee Sutthivaiyakit | วิทยานิพนธ์/Thesis |
Pakawadee Sutthivaiyakit