Impact of clonal origin of hevea brasiliensis tree on the protein composition of latex and dry rubber

Name: Surattiya srisomboon

LCSH: Kasetsart University -- Theses. M.S. (Biotechnology) 2021

Classification :.LCCS: QK495.E9

LCSH: Kasetsart University. -- Department of Biotechnology -- Theses

LCSH: Hevea brasiliensis

LCSH: Rubber

LCSH: Latex

Abstract: Latex from Hevea brasiliensis and latex-derived natural rubber (NR) cannot be replaced by synthetic rubber because of its superior properties. However, the lack of consistency of NR quality represents an important drawback. Non-isoprene components are assumed to be involved in both superior properties and non-consistency of NR quality. This work aimed to characterize proteins, one of the most important non-isoprene components, from both latex and NR to evaluate the impact of clonal origin on protein content/compositions. Latex was collected at two periods: high and low latex production periods. Primarily, protein extraction methods from latex and NR (USS) were developed. The optimal protein extraction methods were further applied to latex and NR (mini-ADS) samples prepared from 5 Hevea clones (RRIM600, PB235, RRIT251, BPM24 and GT1). Proteins were extracted from latex by aqueous buffer while protein extraction from NR was more challenging and required NR solubilization in organic solvent prior to extraction. Protein extracts were subjected to SDS-PAGE electrophoresis and obtained gels were studied by image analysis software to get qualitative and quantitative data. Additional indicators were measured including nitrogen (N) content, DRC, TSC, P0, P30 and PRI. Correlations between indicators were highlighted: increasing N content of latex resulted in higher N contents in film and rubber as well as higher PRI. However, no correlation was observed between DRC and N content of latex. Higher N content in rubber resulted in higher PRI. Interestingly, decreasing DRC of latex induced higher nitrogen contents in film and rubber. For both latex and mini-ADS samples from clones BPM24, GT1 and RRIT251, SDS-PAGE patterns were similar while those of RRIM600 and PB235 clones were slightly different between seasons. As a conclusion, various protein content of latex was shown to affect rubber properties (PRI). However, no major qualitative difference between proteins patterns of the 5 clones was observed. Thus, it was proposed that latex proteins most importantly affect rubber properties by their quantitative content more than by their qualitative composition.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Kittipong Rattanaporn

Role: Thesis Advisor

Name: Siriluck Liengprayoon

Role: Thesis CO-Advisor

Name: Celine Bottier

Role: Thesis CO-Advisor

Name: Laurent Vaysse

Role: Thesis CO-Advisor

Created: 2021

Modified: 2025-07-10

Issued: 2025-07-10

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/surattiya-sri-all.pdf

CallNumber: QK495.E9 .S87

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biotechnology

Grantor: Kasetsart University

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	surattiya-sri-all.pdf	3.78 MB

ใช้เวลา

0.040488 วินาที