Identification of key genes in Pasteurella multocida associated with hyaluronic acid biosynthetic pathway using genome-scale metabolic models for hyaluronic acid production in escherichia coli

Name: Pailin Pasomboon

LCSH: Kasetsart University -- Theses. M.S. (Genetics) 2021

Classification :.LCCS: QR82.P25

LCSH: Kasetsart University. -- Department of Genetics -- Theses

LCSH: Pasteurella multocida

LCSH: Escherichia coli

LCSH: Hyaluronic acid -- Synthesis

LCSH: Genes

Abstract: Pasteurella multocida produces different capsular polysaccharides according to their capsular types (A, B, D, E, and F), especially capsular type A that can produce hyaluronic acid (HA) as a component in certain capsular types of this bacterium. This study intends to 1) identify genes associated with a hyaluronic acid biosynthetic pathway in P. multocida by sequence and structure analysis and 2) modify a genomescale metabolic model to predict hyaluronic acid productivity under different carbon sources and nitrogen supplement in the Escherichia coli metabolic model by the addition of the P. multocida HA biosynthetic genes. Comparison of nine HA biosynthetic genes from eleven P. multocida strains (A:B:D:F = 8:1:1:1) compared with those of other organisms showed a high level of within-species similarity (98– 99%). The only hyaD of capsular type A:3 and capsular type F differed significantly from other strains (82%). Analysis of amino acid patterns together with phylogenetic results showed closely–related HA biosynthetic genes of types A and D compared to those of types B and F. However, the capsular type F was similar to type A:3. Protein structural analysis supported structural similarities of the encoded enzymes between the capsular types A, B, D, and F, except Glck, Pgm, GlmU and HyaD. Reconstruction of a metabolic genome-scale in E. coli by adding two enzymes (GLMU2 and HYAD) from P. multocida for improving the HA production under specifying the amount of carbon sources and nitrogen supplements. Result revealed that threonine and aspartate supplement was beneficial for the HA production. Our bioinformatics analyses proposed that variations observed within these genes and genome-scale metabolic model could be useful for improving the HA production.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Teerasak E-kobon

Role: Thesis Advisor

Name: Pramote Chumnanpuen

Role: Thesis CO-Advisor

Created: 2021

Modified: 2568-07-10

Issued: 2025-07-10

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2564/pailin-pas-all.pdf

CallNumber: QR82.P25 .P35

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Genetics

Grantor: Kasetsart University

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