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Pattama Sonsaka. The inserted position analysis in transgenic orchid genome possess antisense CP-AC01 Gene. Master's Degree(Agricultural Biotechnology). Kasetsart University. Office of the University Library. : Kasetsart University, 2020.
The inserted position analysis in transgenic orchid genome possess antisense CP-AC01 Gene
Name: Pattama Sonsaka
Classification :.LCCS: QK495.O64
LCSH:
Dendrobium
-- Genetics
LCSH:
Agrobacterium
Abstract:
Plant genetic transformation via Agrobacterium-mediated transformation is an effective method and widely adopted. The transgene is randomly inserted into host plant genome, therefore it is difficult to predict the accurately integration sites. In order to get the approval of genetically modified (GM) plants for commercialization and release, biosafety assessments are required. This evaluation requires molecular characterisation of transgene insertion and their flanking sequences. In this study, the analysis of the flanking sequences of LB T-DNA inserted position of transgenic Dendrobium Sonia Earsakul' orchid line AE1 possess antisense CP-ACO1 gene in T-DNA that resulted in the lower of ethylene production and a longer vase life of flowers was performed. Inverse Polymerase Chain Reaction (IPCR) technique was used to amplify the LB flanking sequences from EcoRI, SacI and SphI DNA libraries. The sequences obtained from SphI and SacI libraries showed high similarity. The sequence alignment with original LB T-DNA revealed the 270 bp LB T-DNA sequence and 167 bp deletion from the LB inner end. BLASTN analysis showed 70% similarity (217 out of 310 bp) with the 3UTR of MADS8 gene in Erycina pusilla and 69% similarity (501 out of 724 bp) with Dendrobium catenatum. The existence of the LB T-DNAs position and its own flanking sequence in orchid genome was confirmed.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Sermsiri Chanprame
Role:
Thesis Advisor
Name: Sontichai Chanprame
Role:
Thesis CO-Advisor
Name: Parichart Burns
Role:
Thesis CO-Advisor
Created:
2020
Modified:
2025-07-10
Issued:
2025-07-10
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2563/pattama-son-all.pdf
CallNumber:
QK495.O64 .P35
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Agricultural Biotechnology
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.039761 วินาที
Pattama Sonsaka
| Title | Contributor | Type |
|---|---|---|
| The inserted position analysis in transgenic orchid genome possess antisense CP-AC01 Gene
มหาวิทยาลัยเกษตรศาสตร์ Pattama Sonsaka | Sermsiri Chanprame | วิทยานิพนธ์/Thesis |
Sermsiri Chanprame
Sontichai Chanprame
Parichart Burns