Study on the albumin/starch scaffold for biocompatibility of living cells

Name: Thridsawan Prasopdee

LCSH: Kasetsart University -- Theses. M.S. (Nanomaterials Science) 2018

Classification :.LCCS: R857.M3

LCSH: Kasetsart University. -- Department of Materials Science -- Theses

LCSH: Biomedical materials

LCSH: Tissue scaffolds

LCSH: Biocompatibility

LCSH: Albumins

LCSH: Starch

Abstract: Tissue engineering involves the use of a “scaffold” combining with cells and biologically active molecules into functional tissues in order to generate new viable tissue for tissue formation. Bovine serum albumin (BSA) and cassava starch (starch) have been used as scaffold biomaterials using the freeze-drying method in this research. The BSA/starch scaffold can be fabricated by freeze-drying method while the BSA alone cannot be fabricated due to the fact that it could not maintain a rigid structure. The albumin/starch scaffolds showed the porous structure and provided the characteristics bands of albumin via FTIR study. The alcohol treatment has the ability in transforming the protein structure in the scaffold. SAXS/WAXS data indicated that the BSA molecules are uniformly dispersed within the starch framework and thereby the characteristic size of the scaffold. The BSA/starch scaffold with methanol treatment has better mechanical property compared to that with ethanol treatment. Cytotoxicity by MTT assay showed that the percentage of cell viability of the BSA/starch scaffold in every conditions are higher than 75%. So, the BSA/starch scaffolds without treatment, with ethanol treatment and with methanol treatment are non-toxic to living cells. All these results indicate that the BSA/starch scaffolds could be used for tissue formation in tissue engineering.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Wirasak Smitthipong

Role: Thesis Advisor

Name: Chomdao Sinthuvanich

Role: Thesis CO-Advisor

Created: 2018

Modified: 2025-07-09

Issued: 2025-07-09

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/thridsawan-pra-all.pdf

CallNumber: R857.M3 .T47

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Nanomaterials Science

Grantor: Kasetsart University

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