Biodegradation of terephthalic acid in the presence of ethylene glycol

Name: Nittikarn Suwannawat

LCSH: Kasetsart University -- Theses. M.S. (Biotechnology) 2018

Classification :.LCCS: TP248.T46

LCSH: Kasetsart University. -- Department of Biotechnology -- Theses

LCSH: Terephthalic acid

LCSH: Ethylene glycols

LCSH: Biodegradation

Abstract: Polyester fiber production and the textile industry are vitally important for the Thai economy. During polyester textile manufacturing, large volumes of wastewater are generated with terephthalic acid (TA) and ethylene glycol (EG) as the main pollutants. Terephthalic acid is extremely harmful to human health and environmental biodegradation of this chemical requires extensive treatment of polyester textile wastewater. Eighty-seven isolates were collected and screened from soil around the wastewater basin of a polyester textile manufacturer in Thailand, based on their capability of growing aerobically on TA as the sole carbon and energy source. Isolate number 2 was determined as the most efficient strain that degraded up to 10 g/L of TA within 120 h with TA-degrading ability of 99.6%. This isolate was identified as Rhodococcus biphenylivorans according to its morphological and biochemical properties and 16S rRNA sequence analysis. The presence of inorganic nitrogen sources demonstrated higher potential to degrade TA than using organic nitrogen sources. Among degrading parameters, shaking speed was found to be the most important factor regarding TA-degrading ability after cultivation for 30 h. Optimal conditions were 30ºC, pH 7.0 and 200 rpm shaking speed. When the strain was cultured on minimal salt medium containing both TA and EG as a mixture of carbon and energy sources, TA and EG were consumed simultaneously, with EG degradation higher than in the presence of only EG as the only substrate. Growth kinetics and TA degradation of R. biphenylivorans weredescribed using the Monod equation. Specific growth rate of the strain and specific TA consumption rate increased at higher TA concentration. Results suggested that R. biphenylivoranshad greatpotential for the bioremediation of TA in polyester textile wastewater.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Werasit Sanpamongkolchai

Role: Thesis Advisor

Name: Pramuk Parakulsuksatid

Role: Thesis CO-Advisor

Name: Nuttakan Nitayapat

Role: Thesis CO-Advisor

Created: 2018

Modified: 2025-07-08

Issued: 2025-07-08

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2561/nittikarn-suw-all.pdf

CallNumber: TP248.T46 .N58

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biotechnology

Grantor: Kasetsart University

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