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Siriorn Anootthato. Development of protein hydrolysate from silkworm pupae (Bombyx mori). Master's Degree(Agro-Industrial Product Development). Kasetsart University. Office of the University Library. : Kasetsart University, 2019.
Development of protein hydrolysate from silkworm pupae (Bombyx mori)
Name: Siriorn Anootthato
Classification :.LCCS: QP609.P78
LCSH:
Protein hydrolysates
LCSH:
Bombyx mori
-- Utilization
Abstract:
The objective of this study was to investigate the effect of enzymatic hydrolysis on physicochemical and functional properties of silkworm pupae (Bombyx mori) protein hydrolysate. Antioxidant activity and functional properties influenced by its composition, molecular weight and amino acids were determined. Production of protein hydrolysate from silkworm pupae, using 2 enzymes (Alcalase and Neutrase) were studied. Enzymatic hydrolysis increased protein content from 56.64 % (dry basis) of raw silkworm pupae to 79.82 % (dry basis) of Alcalase hydrolysis. The highest degree of hydrolysis (DH) was achieved by Alcalase hydrolysis (9.61%) while DH from Neutrase hydrolysis was 2.91%. Total amino acid of enzymatic hydrolysis was increased, compared with raw and dried samples. Water solubility index of all enzymatic hydrolysis sample was significantly (p<0.05) different from the dried and defatted samples.
The molecular weight of Alcalase treatment was less than that using the Neutrase treatment. Both enzymatic hydrolysis samples had the lower molecular weight than non-hydrolysis samples. Antioxidant activity was determined by DPPH (IC50) and FRAP. Enzymatic hydrolysis could improve the antioxidant activity from 0.52 mg/ml (IC50) and 1.28 μmol of Fe/g sample (FRAP) in raw sample to 0.06-0.14 mg/ml (IC50) and 4.43-9.20 μmol of Fe/g sample (FRAP) in enzymatic hydrolysis samples. Functional properties of protein hydrolysate such as nitrogen solubility (NS), emulsifying activity index (EAI), emulsifying stability index (ESI), foam capacity (FC) and foam stability (FS) were determined. Hydrolysis of silkworm pupae (Bombyx mori) by Alcalase and Neutrase could improve nitrogen solubility, foam capacity and foam stability.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Nantawan Therdthai
Role:
Thesis Advisor
Name: Pitiporn Ritthiruangdej
Role:
Thesis CO-Advisor
Created:
2019
Modified:
2025-07-04
Issued:
2025-07-04
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2562/siriorn-ano-all.pdf
CallNumber:
QP609.P78 .S57
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Agro-Industrial Product Development
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.036538 วินาที
Siriorn Anootthato
| Title | Contributor | Type |
|---|---|---|
| Development of protein hydrolysate from silkworm pupae (Bombyx mori)
มหาวิทยาลัยเกษตรศาสตร์ Siriorn Anootthato | Nantawan Therdthai | วิทยานิพนธ์/Thesis |
Nantawan Therdthai
| Title | Creator | Type and Date Create |
|---|---|---|
| Effect of drying methods on the durian flour properties
มหาวิทยาลัยเกษตรศาสตร์ Nantawan Therdthai ;Weibiao Zhou ;Pisit Dhamvithee | Shitapan Bai-Ngew | วิทยานิพนธ์/Thesis |
| Development of batter from Myanmar Local wheat flour
มหาวิทยาลัยเกษตรศาสตร์ Nantawan Therdthai ;Anuvat Jangchud ;Kamolwan Jangchud | Ei Mon Mon Kyaw | วิทยานิพนธ์/Thesis |
| Development of protein hydrolysate from silkworm pupae (Bombyx mori)
มหาวิทยาลัยเกษตรศาสตร์ Nantawan Therdthai | Siriorn Anootthato | วิทยานิพนธ์/Thesis |
Pitiporn Ritthiruangdej
| Title | Creator | Type and Date Create |
|---|---|---|
| Gluten-free bread with reduced glycemic potential
มหาวิทยาลัยเกษตรศาสตร์ Nantawan Therdthai;Weibiao Zhou;Pitiporn Ritthiruangdej | Chonnikarn Srikanlaya | วิทยานิพนธ์/Thesis |
| Antimicrobial and functional properties of protein extracts from Khai-nam (Wolffia globosa) prepared by ultrasound-assisted extraction
มหาวิทยาลัยเกษตรศาสตร์ Suvimol Charoensiddhi;Pitiporn Ritthiruangdej | Natchaya Duangjarus | วิทยานิพนธ์/Thesis |