Ethanol production and mitochondrial-related gene expression during maize seed deterioration

Name: Chalisa Chaengsakul

LCSH: Kasetsart University -- Theses. M.S. (Agronomy) 2019

Classification :.LCCS: SB191.M2

LCSH: Kasetsart University. -- Department of Agronomy -- Theses

LCSH: Corn -- Seeds

LCSH: Corn -- Seeds -- Quality

LCSH: Corn -- Seeds -- Deterioration

LCSH: Corn -- Storage

LCSH: Gene expression

LCSH: Gene expression -- Analysis

LCSH: Mitochondria -- Gene expression -- Analysis

Abstract: Mitochondrial degradation plays an important role in seed deterioration. The expression of mitochondrial genes and fast ethanol assay were investigated to explain how does it affect seed deterioration during storage. Maize seeds were stored under ambient condition (AC) (at lab bench, ~27°C and 50-80% RH) or controlled condition (CC) (15±1°C and 50±5% RH in low oxygen permeable bag) for 12 months. Seed samples were evaluated using fast ethanol assay and quantitative RT-PCR (qPCR) for mitochondrial-related genes such as; alcohol dehydrogenase (ADH1), alternative oxidase (AOX1), cytochrome c oxidase (COXc) and ATPase. Germination test and accelerated ageing were every 3 monthly measured throughout the storage period. The results revealed that germination of seed stored in AC reduced significantly when compared to CC and germination tended to decrease over storage time. Ethanol production of maize seeds measured by fast ethanol assay increased upon storage time. After 6 months storing, maize seeds under AC produced ethanol approximately 400 µg l-1 compared to 240 µg l-1 in the CC. Ethanol production increased along with the trend of transcription level of ADH1 (increased after natural ageing). Maize seeds were also aged using hot-humid treatment (HHT) at 42ºC in 100%RH for 12, 24 and 48 hours. Maize seeds at 24 hours under HHT produced ethanol more than 12 hours and control (non-treated seed). The transcription level of COXc of maize seed after HHT significantly decreased that followed to timing of the treatment. The mRNA level of ADH1 during storage and HHT increased according to ethanol production. In conclusion, fast ethanol assay and qPCR evaluated in maize seed deterioration and capitalize in evaluation of seed storability.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Damrongvudhi Onwimol

Role: Thesis Advisor

Name: Pasajee Kongsil

Role: Thesis CO-Advisor

Created: 2019

Modified: 2025-07-03

Issued: 2025-07-03

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/chalisa-cha-all.pdf

CallNumber: SB191.M2 .C44

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agronomy

Grantor: Kasetsart University

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