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Nattawut Srisombat. Antioxidant, anti-HIV properties and isolation of betulinic acid of TIO KHON (Cratoxylum formosum subsp pruniflorum (KUrz) gogelein. Master's Degree(Botany). Kasetsart University. Office of the University Library. : Kasetsart University, 2018.
Antioxidant, anti-HIV properties and isolation of betulinic acid of TIO KHON (Cratoxylum formosum subsp pruniflorum (KUrz) gogelein
Name: Nattawut Srisombat
Classification :.LCCS: QK495.G87
LCSH:
Guttiferae
LCSH:
Antioxidants
LCSH:
Betulinic acid
LCSH:
Bioactive compounds
LCSH:
HIV (Viruses)
Abstract:
Cratoxylum formosum subsp. pruniflorum (Kurz) Gogelein have been used as a medicinal plant for treatment of many diseases. In this study, we macerated C. formosums bark with methanol and partitioned with hexane and chloroform. Chloroform bark extract was fractionated using column chromatography technique. Five fractions were obtained (CFA-CFE) and then investigated for antioxidant activities such as total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), free radical scavenging activity (DPPH) and nitric oxide radical scavenging activity (NO) and also anti-HIV-1 reverse transcriptase activity. For antioxidant activity, CFE had the highest value of TPC, TFC and FRAP (44.84±0.05 µg of GAE/g, 580.69±0.78 µg of QE/g and 82.56±0.08 µg of TE/g of dry weight, respectively) and CFB inhibited nitric oxide radical at the lowest concentration at 96.73 µg/mL. However, for anti-HIV-1 reverse transcriptase (RT) activity, the methanolic bark extract, CFC, CFD and CFE showed high inhibitory effect against HIV-1 RT with the value more than 60% of relative inhibition. CFE has showed the highest percent of relative inhibition, among any other fractions and comparable level as the standard drug (Nevirapine). The ranking of antioxidant and anti-HIV-1 RT activities of fractions was CFE followed by CFD, CFC, CFB and CFA, respectively. The correlation of anti-HIV-1 RT and antioxidant activities were correlated with TPC, TFC and FRAP (r=0.97, 0.92 and 0.94, respectively). Betulinic acid was isolated from fraction 52 of chloroform extract. The 1H and 13C NMR data obtained were in accordance with the previous published data. Further isolation of bioactive compounds and evaluation for their antioxidant and anti-HIV activities is needed.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Sutsawat Duangsrisai
Role:
Thesis Advisor
Name: Srunya Vajrodaya
Role:
Thesis CO-Advisor
Created:
2018
Modified:
2025-07-02
Issued:
2025-07-02
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2561/nattawut-sri-all.pdf
CallNumber:
QK495.G87 .N38
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Botany
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.022276 วินาที
Nattawut Srisombat
| Title | Contributor | Type |
|---|---|---|
| Antioxidant, anti-HIV properties and isolation of betulinic acid of TIO KHON (Cratoxylum formosum subsp pruniflorum (KUrz) gogelein
มหาวิทยาลัยเกษตรศาสตร์ Nattawut Srisombat | Sutsawat Duangsrisai Srunya Vajrodaya | วิทยานิพนธ์/Thesis |
Sutsawat Duangsrisai
Srunya Vajrodaya