Monnipa Srimartpirom. Low dose irradiation with modified atmosphere packaging as quarantine treatment of the oriental fruit fly, bactrocera dorsalis (Hendel) (DipteraTephritadae), on Mango. Doctoral Degree(Entomology). Kasetsart University. Office of the University Library. : Kasetsart University, 2018.
Low dose irradiation with modified atmosphere packaging as quarantine treatment of the oriental fruit fly, bactrocera dorsalis (Hendel) (DipteraTephritadae), on Mango
Abstract:
Low dose gamma irradiation with modified atmosphere packaging (MAP) bag against the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) on mango was studied. Sub-lethal radiation doses, dose-response tests, Nam Dok Mai mangoes infested with third-instar larvae of B. dorsalis, wrapped with three different kinds of MAP bags (CF1, FF5, and H34M) or no MAP, were treated with gamma radiation at 0 (control), 30, 60, 90, 120, and 150 Gy. Supplementary experiments, dose-response tests were done using low-irradiation doses at 15, 30, 45, and 60 Gy with H34M bags comparing with no MAP on mortality of B. dorsalis. The use of MAP on infested mangoes significantly increased mortality of B. dorsalis under irradiation treatment. The estimated lethal doses to cause 99% mortality (LD99) for no MAP and MAP (CF1, FF5, and H34M bags) treatments were 58.1, 41.6, 43.8, and 47.4 Gy, respectively. Therefore, MAP acted as an additional stressor rather than providing radioprotection in irradiated B. dorsalis. Effects of low dose-response on the development of B. dorsalis were illustrated at 60-Gy dose with FF5 bag. Measurements of O2 and CO2 concentrations in MAP bags at 48 h post 60-Gy irradiation were lowest in the H34M bag, averaging 7.2 and 8.7%, respectively, while the average O2 and CO2 concentrations in the CF1 bag were 8.6 and 21.2%, and FF5 bag were 9.6 and 26.7%, respectively. Large-scale confirmatory test of mangoes infested with B. dorsalis third instars wrapped with H34M bag, and then irradiated at 150 Gy showed no survival (adult emergence) in 35,000-treated individuals. Commercial use of MAP producing the O2 levels that I observed for mangoes in this study did not reduce the efficacy of the approved 150 Gy quarantine irradiation treatment for B. dorsalis.
Kasetsart University. Office of the University Library