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Watcharakorn Mongkol. Differential gene expression of plasmodium vivax-infected and uninfected anopheles dirus. Doctoral Degree(Genetics). Kasetsart University. Office of the University Library. : Kasetsart University, 2018.
Differential gene expression of plasmodium vivax-infected and uninfected anopheles dirus
Name: Watcharakorn Mongkol
Classification :.LCCS: QL536
LCSH:
Anopheles
LCSH:
Malaria
LCSH:
Gene expression
LCSH:
Plasmodium vivax
Abstract:
Malaria is a public health problem caused by Plasmodium parasites. These parasites are transmitted to human by Anopheles mosquitoes. In Thailand, P. vivax is the predominant species of malaria parasites. An. dirus is a primary vector in the country. Understanding the molecular interaction between the parasite and the mosquito will assist elimination of malaria. In this study, transcriptome sequencing (RNA-seq) was conducted to determine changes in expression of the An. dirus genes upon P. vivax infection at different time points. Three conditions of An. dirus - bloodfed, sugar-fed, and P. vivax infected blood fed, were used to generate transcriptomes. Approximately 85% of An. dirus genes and 25% of P. vivax genes were mapped. Genes with >2-fold change in transcripts among these 3 conditions were identified and confirmed by qRT-PCR. Three up-regulated An. dirus genes - carboxylesterase, cuticular protein RR-2 family (cpr129) and yellow-g - were selected from the RNAseq data at 18 hours post feeding for targeted knockdown by oral dsRNA delivery. The transcription levels of yellow-g and carboxylesterase were significantly reduced. Further experiment is required to confirm whether these two genes are important for the P. vivax development inside the mosquito. If the knockdowns of these two genes could suppress the parasite in mosquitoes, these genes would be novel targets for development of new malaria transmission blocking intervention.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Anchanee Kubera
Role:
Thesis Advisor
Name: Wang Nguitragool
Role:
Thesis CO-Advisor
Created:
2018
Modified:
2025-06-29
Issued:
2025-06-29
วิทยานิพนธ์/Thesis
application/pdf
URL: https://www.lib.ku.ac.th/KUthesis/2561/watcharakorn-mon-all.pdf
CallNumber:
QL536 .W38
eng
DegreeName: Doctor of Philosophy
Level: Doctoral Degree
Descipline: Genetics
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
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0.029036 วินาที
Watcharakorn Mongkol
| Title | Contributor | Type |
|---|---|---|
| Active compounds against anopheles carboxypeptidase B using for potential used in blocking malaria transmission
มหาวิทยาลัยเกษตรศาสตร์ Watcharakorn Mongkol | Anchanee Kubera | วิทยานิพนธ์/Thesis |
| Differential gene expression of plasmodium vivax-infected and uninfected anopheles dirus
มหาวิทยาลัยเกษตรศาสตร์ Watcharakorn Mongkol | Anchanee Kubera Wang Nguitragool | วิทยานิพนธ์/Thesis |
Anchanee Kubera
Wang Nguitragool
| Title | Creator | Type and Date Create |
|---|---|---|
| Differential gene expression of plasmodium vivax-infected and uninfected anopheles dirus
มหาวิทยาลัยเกษตรศาสตร์ Anchanee Kubera;Wang Nguitragool | Watcharakorn Mongkol | วิทยานิพนธ์/Thesis |