Molecular epidemiology of bovine tuberculosis in buffaloes

Name: Nantawan Yatbantoong

LCSH: Kasetsart University -- Dissertations. Ph.D. (Bio-Veterinary Science) 2018

Classification :.LCCS: SF967.T8

LCSH: Kasetsart University. -- Department of Veterinary Medicine -- Dissertations

LCSH: Tuberculosis in cattle

LCSH: Buffaloes -- Diseases

Abstract: This current research studied the molecular epidemiology on bovine tuberculosis in buffaloes. Internal organs from buffaloes tested positive to single intradermal tuberculin test (SIT) for tuberculosis were collected, cultured and identified for the presence of Mycobacterium bovis. Direct identification of bacterial DNA from fresh specimens was also applied using PCR. In addition, comparison of 3 DNA extraction methods from tissue samples was investigated. Genotyping of M. bovis was performed with 12 loci of MIRU-VNTR and then classified by MIRUVNTR plus database. Results revealed that 216 tissue samples were collected from 106 SIT-positive buffaloes. There were only 5.56% (12/ 216 samples) from 2 locations could be isolated from lung specimens but none of other samples from neither liver nor lymph node provided any isolates. Digesting tissue samples with lysozyme prior to DNA extraction was superior to the other methods The twelve MIRU- VNTR profile from 12 lung’s culture samples in this study could not be used to classified strain of Mycobacterial spp. via MIRU- VNTR plus tool. It can be concluded that these 12 MIRU- VNTR loci was not suitable profile for buffaloes in Thailand because of insufficient discriminatory power and limited data of buffalo’s genetic available in online database. In conclusion, this study was the first report that MIRU-VNTR technique was used to analyze DNA polymorphism among M. bovis isolates from buffaloes. New and interesting information provided was buffaloes in the same area had different genetic strains of M. bovis indicating multiple strains of M. bovis infection with variable genotypes. Further investigation requires large sample size and various sources of specimens to properly represent the buffalo population in Thailand. Moreover, the combination of high resolution power techniques, spoligotyping and MIRU- VNTR typing, are recommended to determine reliable molecular epidemiology for disease investigation.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Theera Rakkwamsuk

Role: Thesis Advisor

Created: 2018

Modified: 2025-06-29

Issued: 2025-06-29

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/0error/2561/nantawan-yat-all.pdf

CallNumber: SF967.T8 .N36

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Bio-Veterinary Science

Grantor: Kasetsart University

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