Supalug Kattakdad. Development of microencapsulated feed for replacing artemia during nursing red bee shrimp (Caridina cantonensis). Doctoral Degree(Aquaculture). Kasetsart University. Office of the University Library. : Kasetsart University, 2018.
Development of microencapsulated feed for replacing artemia during nursing red bee shrimp (Caridina cantonensis)
Abstract:
The popularity, chemical composition and properties of artificial red bee shrimp diet were studied. The top three brands of commercial feed were Shirakura, Benibachi and Borneo Wild. The diets had high protein and ash while a low fat content. The evaluation of the characteristics of the digestive enzymes of red bee shrimp in various pH conditions showed that the larvae shrimp had enzymatic activity significantly lower than the larger shrimp, however, this did not include protease. Protease, amylase, lipase and cellulase activities had optimal activity under alkaline conditions. The trypsin and chymotrypsin activity at neutral to weak alkaline conditions was greater than acidic conditions. The in vitro digestibility of raw materials was also investigated. Red bee shrimp had good diet digestibility in neutral to mid alkaline condition and animal protein materials were appropriate for red bee shrimps feed ingredients being a low fibre and carbohydrate source. Spirulina sp.and squid meal were appropriate protein sources for large shrimp, however, fish meal was a suitable protein source for larvae shrimp. Maltodextrin was the best choice carbohydrate source for feed. Concerning the effect of the processing conditions of a microencapsulated diet (MED), the results showed that total yield, particle size distribution and dietary components of MED depended on the rotational mixer speed, reaction time, sodium alginate levels and their interaction. The processing condition used 5% sodium alginate and was stirred at 500 rpm for 10 min was selected. The physical, chemical and microbiological properties of MED before and after 90 days being stored in modified atmosphere packaging was designated. After being stored the MED showed lower flowability and higher TBA value than before being stored. In addition, the coloration retention was changed after storage. The results of total plate count of MED after storage showed an increase, however, it was still under the limit of acceptability for total bacteria in animal feed in Thailand. The study investigated the effects of MED on red bee shrimp. The commercial feed (COF) and MED were reported to have higher protein digestibility than Artemia shell free (ART). MED was shown to have the highest carbohydrate digestibility, followed by ART and COF. The effect of the diets on growth performance and feed utilization, the shrimps fed with MED showed growth performance in term of final weight, weight gain, specific growth rate, average daily gain, survival rate and feed utilization were better than the other groups. In additions, the shrimps fed with MED over 60 days had the highest protein synthesis capacity (RNA/protein ratio), protease, amylase and lipase activity.
Kasetsart University. Office of the University Library