Multiplex real-time RT-PCR and seed disinfection of pepper chat fruit viroid and culumnea latent viroid in tomato seed

Name: Sukhontip Sombat

LCSH: Kasetsart University -- Dissertations. Ph.D. (Plant Pathology) 2019

Classification :.LCCS: SB608.T75

LCSH: Kasetsart University. -- Department of Plant Pathology -- Dissertations

LCSH: Tomatoes -- Diseases and pests -- Control

LCSH: Tomatoes -- Virus diseases

LCSH: Tomato -- Seeds

LCSH: Pospiviroid infections

LCSH: Viroid diseases of plants

LCSH: Virus diseases of plants

Abstract: Pospiviroid associated with tomato seeds as seed borne pathogens, Pepper chat fruit viroid and Columnea latent viroid are quarantine concerns for the international seed trade. In this study, we developed a multiplex real time RT-PCR method and seed disinfection method for control of the two Pospiviroid species in tomato seeds. This multiplex qRT-PCR could detect CLVd and PCFVd from a single contaminated seed, its sensitivity for plant and seed health assay. The usefulness of newly developed multiplex qRT-PCR for PCFVd and CLVd detection was validated using fieldproduced tomato leaves and commercial seed lot of tomato. Occurrence of seed- borne PCFVd and CLVd using top paper method was found positive in both seed coat and seedling. None of seedlings were infected after grow-out test from 100-650 infected seed at eight weeks post sowing. The efficacy of various seed disinfection methods was evaluated through whole seed, seed coat and seedling. Individual chemical or thermal seed treatments were less effective than combination of chemo-thermal seed treatments for elimination of PCFVd or CLVd on seed coat and in seedling. Thermo therapeutic treatment with 80 oC dry heat treatment for 72 hr was not eliminated two pospiviroid species in whlole seeds and negatively impacts seed germination in varies of tomato varieties. Two effective disinfection methods were evaluated from commercial tomato seed lot and determined using by bioassay with mechanical inoculation of the treated seed extract on indicator plants (Solanum lycopersicum cv. Rutgers). The most effective seed disinfection treatment was the chemo-and thermotherapeutic treatments with 3% sodium hypochlorite combined 50 oC hot water treatment for 25 min which reduced two species of Pospiviroid infection without being toxic to the seeds and minimum impact on germinating capacity of seed.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Kanungnit Reanwarakorn

Role: Thesis Advisor

Created: 2019

Modified: 2025-06-29

Issued: 2025-06-29

วิทยานิพนธ์/Thesis

application/pdf

URL: https://www.lib.ku.ac.th/KUthesis/2562/sukhontip-som-all.pdf

CallNumber: SB608.T75 .S94

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Plant Pathology

Grantor: Kasetsart University

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