Poommate Chomchat.. Reproductive hormone levels in captive and wild female green turtles (Chelonia mydas) in Thailand. Doctoral Degree(Veterinary Clinical Studies). Kasetsart University. Office of the University Library. : Kasetsart University, 2023.
Reproductive hormone levels in captive and wild female green turtles (Chelonia mydas) in Thailand
Abstract:
The relationship between heavy metal exposure and genetic diversity is a complex and multifaceted topic that involves both environmental and genetic factors. The study was composed of 2 experiments which are heavy metal accumulation in green sea turtle at Gulf of Thailand and Andaman sea and also to survey genetic diversity of sea turtles in Thailand. The first study was to survey and compare the amounts of elements in serum of stranded sea turtles. There were 31 stranded sea turtles which were 12 green turtles from Gulf of Thailand and 3 green, 8 hawksbill, and 8 olive ridley turtles from Andaman Sea. Heavy metals in serum were dected using Particleinduced X-ray emission (PIXE). The results found that sea turtles from Gulf of Thailand had Ca, Mg, P, S, Se, and Si concentrations significantly higher than those in sea turtles from Andaman Sea. The concentration of Br in sea turtles from Andaman Sea was significantly higher than those in sea turtles from Gulf of Thailand. The serum concentration of Cu in hawksbill and olive ridley turtles were higher than in green turtles. The second study was studied genetic diversity using polymerase chain reaction used LCM15382 and H950 primer in 91 stranded green turtles from Gulf of Thailand. The obtained 384-bp or 770-bp sequences were analyzed for haplotype, clade and haplotype and nucleotide diversities and were used to construct a phylogenetic tree and haplotype network diagram. Results found that in total, 12 (based on 384 bp) or 13 (based on 770 bp) haplotypes and two clades (clades VII and VIII) were identified. These 2 topics are the leading experiments of detection heavy metals in alive sea turtles and also study genectic diversity of sea turtles in Thailand which are endangered speicies in the world. The monitoring of important elements in sea turtles may be used as a tool to assess the levels of pollution in marine ecosystems and may be reflection to human beings.
Kasetsart University. Office of the University Library