Development of nile and red tilapia broodstock free of tilapia lake virus infection

Name: Jidapa Yamkasem.

LCSH: Kasetsart University -- Dissertations. Ph.D. (Animal Health and Biomedical Sciences) 2023.

Classification :.LCCS: SH179.T35

LCSH: Kasetsart University. -- Faculty of Veterinary Medicine -- Dissertations.

LCSH: Nile tilapia -- Diseases.

LCSH: Nile tilapia -- Pathogens.

LCSH: Nile tilapia -- Diseases -- Climatic factors

LCSH: Tilapia -- Diseases

LCSH: Tilapia -- Pathogens.

Abstract: Tilapia Lake Virus (TiLV) poses a significant threat to global tilapia aquaculture, causing mass morbidity and mortality in tilapia populations. While horizontal transmission of TiLV has been established through cohabitation experiments, the potential for vertical transmission from infected broodstock to progeny remains unclear. The first study confirmed natural outbreaks of TiLV in broodstock and fry in two tilapia hatcheries, detecting TiLV genomic RNA in the liver and reproductive organs of both naturally infected and experimentally challenged fish. In situ hybridization confirmed TiLV presence in the ovary and testis of infected challenged fish, suggesting possible vertical transmission. To mitigate TiLV spread, it is crucial to establish the infection status of source farms before moving live tilapia. However, individual testing for TiLV can be challenging due to cost and labor constraints. In a second study, a pooling technique was evaluated for TiLV detection, finding that a tissue pool from 5 or 10 individual fish was sufficient for detection. This method can be applied to screen fry for TiLV or for disease surveillance. In a third study, TiLV RNA in water was concentrated using an electronegative charge membrane and detected using reverse transcriptase quantitative PCR (RT-qPCR). The virus lost infectivity in E-11 cells after one day in all tested water, despite the genomic material remaining detectable for up to 14 days. These results can be applied to enhance biosecurity practices in tilapia farms by recommending disinfection or resting of reservoir water for three to five days before stocking tilapia to limit TiLV spread. In conclusion, extensive studies offer a robust strategy to strengthen biosecurity measures against TiLV, providing tools to disrupt the spread of TiLV and enhance the strength of farms' biosecurity, fostering a promising and thriving future for global tilapia aquaculture.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Win Surachetpong

Role: Thesis Advisor

Created: 2023

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: http://www.lib.ku.ac.th/KUthesis/2566/jidapa-yamk-all.pdf

CallNumber: SH179.T35.J53

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Animal Health and Biomedical Science

Grantor: Kasetsart University

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