Evaluation of physicochemical and biological properties of Zirconia-alumina-silicate-based composite materials for bone tissue engineering

Name: Tanawut Rittidach

LCSH: Kasetsart University -- Dissertations. Ph.D. (Physics) 2023.

Classification :.LCCS: QC173.4.C63

LCSH: Kasetsart University. -- Department of Physics -- Dissertations.

LCSH: Biomedical engineering.

LCSH: Ceramic materials.

LCSH: Tissue engineering.

LCSH: Tissue scaffolds.

LCSH: Bone regeneration.

Abstract: Novel bioceramic materials based- bioactive scaffolds with improved mechanical properties, biocompatibility, in vivo study and structural support for bone tissue are being developed for the treatment of bone defects. The aim of this research has synthesized bioceramic materials, zirconia-mullite (2ZrO2·[3Al2O3·2SiO2], ZAS), and bioactive materials such as biphasic calcium phosphate (BCP) and diopside (CaMgSi2O6, CMS). Initially, we have synthesized bioactive scaffolds consisting of BCP and ZAS (BCPZAS) combined with polymers matrix of polycaprolactone (PCL)-alginate (Alg)-chitosan (Chi) (BCPZAS@Chi/Alg-PCL). A blending technique prepared the composite material scaffolds. The microstructure, mechanical, bioactivity and in vitro biological properties with different ratios of BCP to ZAS of 1:0, 3:1, 1:1, 1:3 and 0:1 wt% in polymer matrix were analyzed. Microstructure analysis showed successful incorporation of the BCPZAS particles with an even distribution of them within the polymer matrix. The mechanical properties gradually decreased with increasing the ratio of ZAS particles in the scaffolds. Bioactivity test, the BCPZAS@Chi/Alg-PCL composite could induce apatite formation in simulate body fluid (SBF). In-vitro experiment using UMR-106 osteoblast-like cells on BCPZAS@Chi/Alg-PCL composite scaffold showed cell attachment to the scaffolds with proliferation. Moreover, ZAS bioceramic utilization in tissue engineering was mixed with CMS bioactive materials. The physiochemical characterization, in-vitro properties, antimicrobial activity and in-vivo screening toxicity study by zebrafish model of different ratios of ZAS-based composites as a function of CMS contents of 0, 25, 50, 75 and 100 wt% were evaluated. Physical characterizations showed the functional groups, microstructure, pore size and morphology of the ZASCMS scaffolds and well-interconnected particles. The mechanical properties of ZASCMS75, 75wt% CMS, showed the highest compressive strength and modulus. In addition, the composite scaffold exhibited good affinity for osteoblast-like cell adhesion, growth and proliferation. Moreover, the ZASCMS composite scaffolds exhibit antibacterial properties. In vivo performance on embryonic zebrafish studies showed that the synthesized ZAS, CMS and ZASCMS50 composite particles are non-toxic based on the measurements of essential parameters such as survivability, hatching rate and morphological embryo. Therefore, the ZAS bioceramic basedbioactive, which are BCP and CMS, biocomposite materials would be a promising material for potential practical application in bone tissue engineering.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Weeraphat Pon-On

Role: Thesis Advisor

Name: Sutee Boonchuay

Role: Thesis Co-Advisor

Name: Chatchawal Wongchoosuk

Role: Thesis Co-Advisor

Created: 2566

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: http://www.lib.ku.ac.th/KUthesis/2566/tanawut-ritt-all.pdf

CallNumber: QC173.4.C63.T36

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Physics

Grantor: Kasetsart University

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