Expression of Ethylene biosynthesis, Ethylene receptor and Ethylene signaling genes associated with Ethylene action during senescence of cut Dendrobium flowers

Name: Kanokwan Thanomchit.

LCSH: Kasetsart University -- Dissertations. Ph.D. (Horticulture) 2023.

Classification :.LCCS: QK495.O64

LCSH: Kasetsart University. -- Department of Horticulture -- Dissertations.

LCSH: Dendrobium -- Genetic

LCSH: Dendrobium -- Breeding.

LCSH: Ethylene -- Synthesis.

Abstract: Dendrobium flowers typically exhibit sensitivity to ethylene, leading to premature senescence. Specifically, Dendrobium 'Khao Chaimongkol' demonstrates heightened sensitivity to ethylene. A study was conducted to explore the effects of both exogenous and endogenous ethylene on premature senescence by investigating visible symptoms related to ethylene biosynthesis, receptors, and signal transduction genes. The inflorescences were treated with exogenous ethylene, 1- MCP, and 1-MCP followed by ethylene. The open florets treated with ethylene rapidly displayed senescence symptoms, with the initial visible signs being venation and drooping. The expression of ethylene receptor and signaling genes was upregulated in floral buds subjected to ethylene treatment. 1-MCP treatment slightly inhibited the expressions of the ethylene biosynthesis gene, DenACO1, the ethylene receptor DenERS1, and three signal transduction genes, DenCTR1, DenEIL1, and DenERF1 in floral buds. Notably, 1-MCP treatment did not affect the expression of DenACS1 in open florets. The results suggest that ethylene and 1-MCP play a role in the differential expression of ethylene biosynthesis, receptor, and signal transduction genes at the different developmental satges of Dendrobium cut flowers. Following successful pollination, Dendrobium flowers exhibited rapid senescence due to the effect of endogenous ethylene. In the case of compatible pollination, there was an accelerated production of ethylene and a swifter development of senescence symptoms compared to nonpollinated or incompatible pollinia. The expression of the ethylene receptor gene DenERS1 and signaling genes DenEIL1 and DenERF1 showed differential spatial regulation with greater expression in the perianth than in the column plus ovary following compatible pollination. Compatible pollinia increased ethylene production concomitant with premature senescence and suppressed the ethylene receptor gene DenERS1, whereas incompatible pollinia did not stimulate ethylene production nor induced premature senescence but increased higher expression of DenERS1 in both the perianth and the column plus ovary. These results suggest that compatible pollinia induced a negative regulation of DenERS1 which may play an important role in ethylene perception, and in modulating ethylene signaling transduction during pollinia-induced flower senescence. In conclusion, the acceleration of premature senescence and gene expression in Dendrobium florets by both exogenous and endogenous ethylene depends on the stage of flower development and the specific organ.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Anchaya Mongkolchaiyaphruek

Role: Thesis Advisor

Name: Wachiraya Imsabai

Role: Thesis Co-Advisor

Name: Parichart Burns

Role: Thesis Co-Advisor

Name: Poonpipope Kasemsap

Role: Thesis Co-Advisor

Created: 2023

Modified: 2025-06-24

Issued: 2025-06-24

วิทยานิพนธ์/Thesis

วิทยานิพนธ์/Thesis

application/pdf

URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2566/kanokwan-tha-all.pdf

CallNumber: QK495.O64.K36

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Horticulture

Grantor: Kasetsart University

©copyrights Kasetsart University

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