Optimization for High-Yield Adenosine Production from Cordyceps militaris in Submerged Fermentation

Name: Chuenprasert N

Address: 333 Moo1, Thasud, Muang, Chiang Rai 57100

Organization : Center of Excellence in Fungal Research, Mae Fah Luang University

Email : library@mfu.ac.th

Name: Yodsuwan N

Name: Wei T

Name: Chamyuang S

keyword: Adenosine

ThaSH: Adenosine

; Cordyceps militaris

ThaSH: Cordyceps

; Process Optimization

ThaSH: Taguchi methods (Quality control)

; Submerged Fermentation

; Taguchi Method

Abstract: Cordyceps militaris, a prized medicinal fungus in East Asian traditional medicine, is renowned for its bioactive compound, adenosine. This compound exhibits diverse pharmacological properties, including anti-aging, skin regeneration, wrinkle reduction, and hair growth promotion. To optimize adenosine production, we employed a Taguchi experimental design to evaluate the impact of key factors with the following conditions: fungal strains (C. militaris SH01, C. militaris ATCC 34165, and hybrid strain of C. militaris SH01 and C. militaris ATCC 34165), glucose concentration (20, 40, and 60 g/L), yeast extract concentration (5, 10, and 20 g/L), and initial pH (4.0, 5.5, and 7.0). The optimization experiments were carried out in 250-mL shaking flasks at 110 rpm at 25 °C. Among the tested strains, C. militaris SH01 demonstrated superior adenosine production under predicted optimal conditions from the Taguchi method (Qualitek-4 software): 40 g/L glucose, 20 g/L yeast extract, and an initial pH of 4.0. This resulted in a maximum adenosine yield of 8.662±0.269 mg/g and a productivity of 2.495±0.077 mg/g.d, surpassing the predicted values by 44%. However, when scaled up to a 5-L shaking flask, adenosine productivity decreased to 1.849±0.094 mg/g.d. Our findings provide valuable insights for enhancing adenosine production from C. militaris, paving the way for its potential application in various industries, including healthcare, food, and cosmetics.

Mae Fah Luang University. Learning Resources and Educational Media Centre

Address: CHIANG RAI

Email: library@mfu.ac.th

Modified: 2025-06-19

Issued: 2025-06-19

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BibliograpyCitation : Asian Journal of Mycology (AJOM). Vol.8, No.1 (January - June 2025): p.141–155

Asian Journal of Mycology (AJOM). Vol.8, No.1 (January - June 2025): p.141–155

eng

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