Fungal endophytes associated with actinorhizal plant species, Elaeagnus latifolia (Elaeagnaceae) and evaluating their antagonistic potential against grey blight disease in tea [causal agent: Pestalotiopsis theae] in North East India

Name: Handique L

Address: 333 Moo1, Thasud, Muang, Chiang Rai 57100

Organization : Center of Excellence in Fungal Research, Mae Fah Luang University

Email : library@mfu.ac.th

Name: Parkash V

Name: Bhattacharyya PN

Name: Sarma B

keyword: Bioactive molecules

ThaSH: Fungi

; Culture-based approach

ThaSH: Fungi -- Biodiversity

; Endophytic fungal diversity

ThaSH: Endophytic fungi

; Plant growth-promoting traits

ThaSH: Plants, Protection of

; Plant protection

; Species richness and diversity indices

Abstract: The current investigation explores the antagonistic potential of endophytic fungal diversity associated with Elaeagnus latifolia L., an actinorhizal plant species of North-east India against Pestalotiopsis theae, the causal agent of grey blight disease in tea. A total of 17 endophytic fungal isolates belonging to 14 different families and three phyla were isolated from various parts of the target plant species. The fungal isolates were characterized using a culture-based approach and microscopic tools and techniques, with particular emphasis on colony shape, size, spore formation characteristics, and fruiting body features. Nigrospora sp. showed the highest species density (0.5) amongst all of the fungal isolates. The highest isolation frequency (67%) was observed for Fusarium sp., Nigrospora sp., Penicillium chrysogenum and Rhizopus sp. A higher percentage of fungal isolates were obtained from the roots and stems (47% each) compared to leaves (29%). The maximum species richness and diversity indices were observed in stems (15.0), while the minimum values were found in leaves (9.0). The stem had the highest Shannon and Simpson diversity indices (2.02 and 0.860, respectively), followed by the root (1.979 and 0.847, respectively) and the leaves with the lowest values being 1.494 and 0.75, respectively. Among the fungal isolates tested for plant growth-promoting traits, such as phosphate solubilization, starch hydrolysis and efficiency in zinc solubilization, the isolate EF09 showed positive responses for all the tested parameters. The isolate EF09 showed maximum antifungal potential (up to 87.1%) against the significant tea pathogen, P. theae, in poisoned food technique in vitro. As there exists limited research related to hitherto-unexplored fungal endophytes associated with endemic plant species like E. latifolia, the current investigation holds sufficient promises in the isolation of novel bioactive molecules from these microbial isolates that might be useful in agriculture to develop sustainable strategies in plant protection in tea.

Mae Fah Luang University. Learning Resources and Educational Media Centre

Address: CHIANG RAI

Email: library@mfu.ac.th

Modified: 2024-12-11

Issued: 2024-12-11

บทความ/Article

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Asian Journal of Mycology (AJOM), Vol.7 No.2 (2024): p68-89

eng

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