Antibacterial effects of Syzygium aromaticum essential oil (clove oil) against normal flora on the skin

Name: Nitchakarn Phimthong

Organization : Chiang Mai University. Faculty of Medicine

Name: Jomkhwan Meerak

Organization : Chiang Mai University. Faculty of Science

Name: Churdsak Jaikang

Organization : Chiang Mai University. Faculty of Medicine

Name: Nitwara Wikan

Organization : Chiang Mai University. Faculty of Medicine

Name: Wutigri Nimlamoon

Organization : Chiang Mai University. Faculty of Medicine

Name: Saranyapin Potikanond

Organization : Chiang Mai University. Faculty of Medicine

Email : saranyapin.p@cmu.ac.th

keyword: Clove oil

LCSH: Clove tree

; Skin microbiome

LCSH: Essences and essential oils -- Therapeutic use

; Normal flora (Microbiology)

LCSH: Antibacterial agents

Abstract: Central line associated bloodstream infection (CLABSI) is an infection that occurs after the insertion of central line into patients. Most two common microorganisms caused CLABSI are Staphylococcus aureus and S. epidermidis which represent as normal flora on human skin. To prevent this event, chlorhexidine gluconate (CHG) is used to inhibit the growth of normal flora bacteria around the insertion site. However, some patients are allergic to CHG, and cause rash and itching. Therefore, changing an antiseptic agent in the dressing from CHG to other antibacterial agents can be beneficial to patients who have an allergy to CHG. Clove oil is an essential oil from clove bud (Syzygium aromaticum (L.) Merr. & L.M. Perry) and has been reported about its antimicrobial activity. This research aimed to evaluate the antibacterial activity of clove oil against S. aureus ATCC 25923 and S. epidermidis ATCC 12228. Clove oil was obtained by hydrodistillation, and major composition was determined by using gas chromatography coupled with mass spectrometry. From the chromatogram, eugenol was found as major component (40% v/v) Antibacterial activity was observed by using Kirby-Bauer disc diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination. Clove oil demonstrated MIC value against S. aureus and S. epidermidis as 1% and 0.5% v/v, respectively. For MBC value against S. aureus ATCC 25923 and S. epidermidis ATCC 12228 is 1% v/v. Time-kill analysis showed that there was a reduction after an exposure to clove oil at least 5 minutes. In conclusion, clove oil demonstrated the strong antibacterial activity against the normal flora. This result might be developed as antibacterial patch using clove oil as disinfectant agent

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2024

Modified: 2024-12-04

Issued: 2024-12-04

BibliograpyCitation : In Kasetsart University. Faculty of Science. The Pure and Applied Chemistry International Conference 2024 (PACCON 2024) (pp.788-792). Bangkok : Kasetsart University, 2024

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