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Harit Pitakjakpipop. Molecularly imprinted polymer-based biosensor on microneedles for monitoring of glucose and creatinine. Doctoral Degree(Engineering and Technology). Thammasat University. Thammasat University Library. : Thammasat University, 2023.
Molecularly imprinted polymer-based biosensor on microneedles for monitoring of glucose and creatinine
การตรวจวัดปริมาณกลูโคสและครีอะตินีนด้วยเข็มขนาดไมโครเมตรโดยใช้พอลิเมอร์ลอกแบบจับจําเพาะเป็นตัวตรวจวัดทางชีวภาพ
Name: Harit Pitakjakpipop
keyword:
Biosensor
Abstract:
Diabetes mellitus is often diagnosed with blood glucose concentration by
invasive or enzymatic methods, which indicates patients' health. The accuracy of the
enzyme-based electrochemical glucose sensors may be limited due to strip
manufacturing variances, strip storage, and aging. Herein, a microneedle(MN)
molecularly imprinted polymer (MIP) approach was used to develop an electrochemical
non-enzymatic sensor to determine the glucose levels in the interstitial fluid, facilitating
a simple sampling fluid under the skin without bleeding. A cross-shaped microneedles
patch was fabricated via photolithography using mixed acrylated epoxidized soybean
oil and methyl methacrylic acid (AESO/MAA) resin. The gold nanoparticles on the MN
patch were coated using an electroless plating and sputtering method, and MIP
membrane was deposited on the microneedle gold electrodes. 3-
(acrylamido)phenylboronic acid (3APBA) functional monomer, methacrylic acid
(MAA) monomer, ethylene glycol dimethacrylate(EGDMA) cross-linker, and 2,2-
dimethoxy-2-phenylacetophenone (DMPA) initiator were utilized in the presence of a
glucose or creatinine template for the processing of electropolymerization or spin
coating. After the removal of the templets, MIP electrodes created highly selective cavities. Cyclic voltammetry(CV), electrochemical impedance spectroscopy (EIS),
differential pulse voltammetry (DPV), and square wave voltammetry (SWV)
techniques were employed for the electrochemical measurements with potassium
ferrocyanide as a redox probe in phosphate buffer saline. Under optimum conditions,
the microneedle MIP sensor could effectively detect glucose in a working range from
50 to 800 mg/dL, and creatinine in a working range from 0.1 to 2 mg/dL, even underheated
sensor at 50 ºC before testing. Consequently, this work has demonstrated a
simple and effective sensing platform to avoid the limitation of the enzyme-based strip;
it is a promising tool for the future evolution of accurate and reliable non-invasive
diagnosis
Thammasat University. Thammasat University Library
Address:
BANGKOK
Email:
preserv@tu.ac.th
Name: Pakorn Opaprakasit
Role:
advisor
Name: Matsumura, Kazuaki
Role:
co-advisor
Created:
2023
Modified:
2024-07-30
Issued:
2024-07-29
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Doctor of Philosophy
Level: Doctoral Degree
Descipline: Engineering and Technology
Grantor: Thammasat University
©copyrights Thammasat University
RightsAccess:
ใช้เวลา
0.022613 วินาที
Harit Pitakjakpipop
| Title | Contributor | Type |
|---|---|---|
| Molecularly imprinted polymer-based biosensor on microneedles for monitoring of glucose and creatinine
มหาวิทยาลัยธรรมศาสตร์ Harit Pitakjakpipop | Pakorn Opaprakasit Matsumura, Kazuaki | วิทยานิพนธ์/Thesis |
Pakorn Opaprakasit
Matsumura, Kazuaki
| Title | Creator | Type and Date Create |
|---|---|---|
| Molecularly imprinted polymer-based biosensor on microneedles for monitoring of glucose and creatinine
มหาวิทยาลัยธรรมศาสตร์ Pakorn Opaprakasit;Matsumura, Kazuaki | Harit Pitakjakpipop | วิทยานิพนธ์/Thesis |