Evaluation of anti-foodborne bacterial activity, digestive enzyme secretion, and antimicrobial resistant genes as probiotic strains selection for industrial interest

Name: Chayanee Boontun.

Organization : King Mongkut’s University of Technology North Bangkok. Faculty of Applied Science

Name: Savitri Vatanyoopaisarn.

Organization : King Mongkut’s University of Technology North Bangkok. Faculty of Applied Science

Email : savitri.v@sri.kmutnb.ac.th

Name: Vichai Domrongpokkaphan.

Organization : King Mongkut’s University of Technology North Bangkok. Microbial Informatics and Industrial Product of Microbe Research Center

Name: Chantaraporn Phalakornkule.

Organization : King Mongkut’s University of Technology North Bangkok. Faculty of Engineering

Name: Rattapha Chinli.

Organization : Mahidol University. Faculty of Medicine Siriraj Hospital

Name: Pairat Thitisak.

Organization : K.M.P. Biotech Co., Ltd.

Name: Sungwarn Hankla.

Organization : K.M.P. Biotech Co., Ltd.

keyword: Probiotics bacteria.

LCSH: PROBIOTICS.

; Antimicrobial activity.

LCSH: BIFIDOBACTERIUM.

LCSH: DIGESTIVE ENZYMES.

LCSH: LACTOBACILLUS.

Abstract: Beneficial microbes, such as probiotic bacteria, are increasingly in demand in the food and feed industry. Lactic acid bacteria and bifidobacteria are commonly used as commercial probiotics, only a few species have been isolated from Southeast Asia areas. This study employed criteria including antimicrobial activity, the release of digestive enzymes, and the absence of antibiotic-resistant (AMR) genes to screen potential local isolates. The results revealed that 4 out of 16 isolates met these criteria, displaying anti-foodborne bacterial activities and a lack of fifty-one tested AMR genes. Furthermore, the four selected isolates demonstrated the production of extracellular digestive enzymes, including amylase, lipase, protease, β-glucanase, and cellulase, with enzyme indices ranging from 1.09-1.31. Among these isolates, two potential probiotics were identified as Bifidobacterium animalis subsp. lactis (strain H9-01) and Lactobacillus reuteri (strain P4-S03). Importantly, both species are approved for use as food and feed supplements in accordance with Thai regulations. This research outlines an approach for screening potential probiotics for industrial-scale applications.""

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2024

Modified: 2567-06-07

Issued: 2024-06-07

BibliograpyCitation : APPLIED SCIENCE AND ENGINEERING PROGRESS. vol. 17, no. 2 (Apr.-June 2024), p. 1-16.

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