Extraction of collagen from indian squid (Loligo duvauceli) fin wastes in seafood industry

Name: Siripong Somsiriwon

Organization : Chulalongkorn University. Faculty of Engineering

Email : Siripong.somsiriwon@gmail.com

Name: Sorada Kanokpanont

Organization : Chulalongkorn University. Faculty of Engineering

keyword: Loligo duvauceli

ThaSH: Animal extracts

; Indian Squid

ThaSH: Collagen -- Manufacture

; Lyophilization

ThaSH: Seafood industry -- Waste minimization

ThaSH: Freeze-drying

Abstract: Indian squid (Loligo duvauceli) is an important marine product of Thailand. Squid fins have accounted for 12 percent (w/w) of the frozen squid production and considered as wastes of the production line. Dry Indian squid fins generally consist of 4.62 percent moisture, 15.60 percent fat, 77.08 percent protein and 3.03 percent ash (dry weight basis). In this research, acetic solubilized collagen (ASC) was prepared by soaking Indian squid fins in 0.5 M NaOH to remove fats and non-collagenous proteins then they were extracted with 0.5 M acetic acid at 4 degrees. The Liquid and solid were separated by filtration. Liquid was lypophilized, the resulting lypophilized solid is ASC. Acidic Pepsin solubilized collagen (APSC) was prepared by repeat the same method of ASC but adding 1 percent (w/v) pepsin into acetic acid in extraction process. The yield of ASC and APSC was 0.69+0.25 percent and 1.42+0.41 percent respectively (dry weight basis). SDS-PAGE showed the ASC and APSC containing collagen type SQ ([alpha1]2[alpha2]1) similar to mammal type I collagen, which contain alpha1, alpha2 and beta chains, APSC have lower collagen molecular weight. Fourier transform infrared spectra revealed the ASC and APSC have triple-helical structure of collagen by deconvolution which showed secondary structure consist of alpha-helix 24.02 percent and 33.55 percent respectively.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2020

Modified: 2023-12-18

Issued: 2023-12-18

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BibliograpyCitation : In the Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn in association, Thammasat University Faculty of Science and Technology. The Pure and Applied Chemistry International Conference 2020 (PACCON 2020) (CB100-105). Bangkok : Thammasat University

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