Development and validation of a liquid chromatography tandem mass spectrometry method for the determination of 11-nor-9-carboxy-delta(9)-tetrahydrocannabinol in urine

Name: Sirijit Jongjityotin

Organization : Chiang Mai University. Graduate School

Name: Anongphan Junkuy

Organization : Chiang Mai University. Faculty of Medicine

Name: Pongruk Sribanditmongkol

Organization : Chiang Mai University. Faculty of Medicine

Name: Chaturong Kanchai

Organization : Chiang Mai University. Faculty of Medicine

Email : chaturong.k@cmu.ac.th

ThaSH: Mass spectrometry

ThaSH: Liquid chromatography

Abstract: In this study, the LC-MS/MS method was developed and validated to confirm and quantify 11-nor-9-carboxy-delta(9)-tetrahydrocannabinol (THC-COOH); the main metabolite of tetrahydrocannabinol (THC). One milliliter of urine was hydrolyzed with sodium hydroxide and extracted with liquid-liquid extraction. The chromatographic separation was performed on an Infinity Lab Poroshell 120 EC-C18 column with a gradient elution consisting of 0.1 percent formic acid in ultrapure water and acetonitrile. The analyte was monitored in negative ionization multiple reaction monitoring mode. The method was accurate (percentage of relative recovery = 94.55-109.56 percent) and precise (percentage of coefficient variation = 1.33-7.11 percent) with good linearity over the concentration range of 1-1,000 ng/mL. The limit of detection and limit of quantitation were 0.1 and 1 ng/mL, respectively. The extraction recovery ranged from 95.79 to 110.84 percent. There was no matrix effect, carry over, dilution integrity. The THC-COOH in processed sample was stable for 24 hours at room temperature. Twenty-eight THC positive urine samples analysed by immunoassay test kits were determined using this validated method. The THC-COOH concentrations ranged from 5 to 3,000 ng/mL. Seventy-five percent of urine samples contained THC-COOH concentration above the regulatory cutoff for THC-COOH of 50 ng/mL. This method can be used as an applicable analytical tool for the confirmation and quantification of routine urine testing in cannabis users.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2020

Modified: 2023-12-01

Issued: 2023-12-01

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BibliograpyCitation : In the Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn in association, Thammasat University Faculty of Science and Technology. The Pure and Applied Chemistry International Conference 2020 (PACCON 2020) (AC74-79). Bangkok : Thammasat University

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