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Arada Masari. Mapping a major quantitative trait locus controlling seed starch content in mungbean [Vigna radiata (L.) Wilczek]. Doctoral Degree(Plant Breeding). Kasetsart University. Office of the University Library. : Kasetsart University, 2017.
Mapping a major quantitative trait locus controlling seed starch content in mungbean [Vigna radiata (L.) Wilczek]
Name: Arada Masari
Classification :.LCCS: SB317.M85
LCSH:
Genetics
-- Statistical methods
LCSH:
Gene mapping
Abstract:
Mungbean (Vigna radiata) seeds are used for direct consumption by people and are raw materials for sprout, starch and noodle industries in Asia. High-yielding mungbean cultivars with high seed starch content are preferred for the starch and noodle industries. At present, there is only one report on genetic control of seed starch content in mungbean. The objectives of this study were to (i) estimate heritability of the seed starch content in mungbean, and (ii) locate a major quantitative trait locus (QTL) controlling the content. An F2 population of 123 individuals was developed from the cross V6087AG (high seed starch) X V5020BY (low seed starch). Seeds of F2 and F2:3populations were determined for seed starch content. Broad-sense heritability estimated for seed starch content in the F2 and F2:3populations were higher than 80%. Seed starch content showed a relatively high and significant correlation (r = 0.6) with seed weigh in both populations. Bulk segregant analysis using 106 polymorphic SSR markers revealed that only the marker CEDGO92 on mungbean chromosome 8 associated with the starch content. QTL mapping using CEDG092 and 22 newly developed SSR markers from this chromosome confirmed that a major QTL,qSSC8.1, flanked by markers Vr08-SSR113 and Vr08-SSR114 controls seed starch content in both F2 and F2:3populations. qSSC8.1 showed no colocalization with seed weight QTL and explained 12.34-13.84% of total variation of the seed starch content in the two populations. Allele(s) from V6087AG at this QTL increased seed starch content. Vr08-SSR113 and Vr08-SSR114 spanned a genome region of about 385 Kbp and there were 21 annotated genes in this region. This genome region can be used as a target for fine mapping to identify gene controlling seed starch content in mungbean.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Name: Prakit Somta
Role:
Thesis Advisor
Name: Peerasak Srinives
Role:
Thesis Co-Advisor
Created:
2017
Modified:
2023-06-14
Issued:
2023-06-14
วิทยานิพนธ์/Thesis
application/pdf
URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/arada-mas-all.pdf
CallNumber:
SB317.M85.A73
eng
DegreeName: Doctor of Philosophy
Level: Doctoral Degree
Descipline: Plant Breeding
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.029795 วินาที
Arada Masari
| Title | Contributor | Type |
|---|---|---|
| Mapping a major quantitative trait locus controlling seed starch content in mungbean [Vigna radiata (L.) Wilczek]
มหาวิทยาลัยเกษตรศาสตร์ Arada Masari | Prakit Somta Peerasak Srinives | วิทยานิพนธ์/Thesis |
Prakit Somta
Peerasak Srinives