Atcha Boonmee. Isolation of bacteria utilizing glycerol as a sole carbon source for potential production of 1,3-Propanediol production. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2021.
Isolation of bacteria utilizing glycerol as a sole carbon source for potential production of 1,3-Propanediol production
Abstract:
Glycerol is a by-product waste from biodiesel production, which can be
converted by bacteria to other value-added products. One of those products is 1,3-
propanediol, an important substrate for synthesis of biopolymer and many others. This
work aims to isolate bacteria that can utilize glycerol as a sole carbon source and
examine the 1,3-propanediol production ability of those isolates using glycerol as a
substrate. Bacterial isolates were obtained from soil near biodiesel plant by culturing in
mineral salt medium containing glycerol. Growth of selected isolates in medium
containing glycerol as a sole carbon source were monitored and compared with
reference bacterial strains. It was observed that 2 isolates grew better than reference
strains according to optical density value at 600 nm. Both isolates were identified as
Klebsiella pneumonia by 16S rRNA gene sequencing. The isolate 5-3, renamed as K.
pneumonia KKU5 (Kp KKU5) was used for further study. The dhaT gene of Kp KKU5
shared 99 percent identity with those of K. pneumoniae (CP025630.1). To examine the ability
of Kp KKU5 as a 1,3-propanediol producer, its fermentation broth containing glycerol
was analyzed by HPLC. Under batch fermentation condition with 20 g/L initial
glycerol, Kp KKU5 produced 0.375 g/L of 1,3-propanediol after 7 days. Despite its low
production of 1,3-propanediol, we have demonstrated that Kp KKU5 was able to utilize
glycerol as a sole carbon source. This study might provide some useful information to
contribute in the research area of 1,3-propanediol production, which adds value to
waste glycerol from biodiesel production.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2021
Modified:
2023-06-09
Issued:
2023-06-09
BibliograpyCitation :
In Thai Society for Biotechnology. Thai Society for Biotechnology and International Conference Online (TSB Online 2021) (pp.641-651). Bangkok : Thai Society for Biotechnology