Isolation of bacteria utilizing glycerol as a sole carbon source for potential production of 1,3-Propanediol production

Name: Nittiyaphorn Sotapong

Organization : Khon Kaen University. Faculty of Science, Khon Kaen University. Graduated School

Name: Mallika Boonmee Kongkeitkajorn

Organization : Khon Kaen University. Faculty of Technology, Khon Kaen University. Fermentation Research Center for Value Added Agricultural Products

Name: Atcha Boonmee

Organization : Khon Kaen University. Faculty of Science

Email : atcha@kku.ac.th

keyword: Propanediol oxidoreductase

ThaSH: Biodiesel fuels -- Production

ThaSH: Glycerin

ThaSH: Klebsiella pneumoniae

Abstract: Glycerol is a by-product waste from biodiesel production, which can be converted by bacteria to other value-added products. One of those products is 1,3- propanediol, an important substrate for synthesis of biopolymer and many others. This work aims to isolate bacteria that can utilize glycerol as a sole carbon source and examine the 1,3-propanediol production ability of those isolates using glycerol as a substrate. Bacterial isolates were obtained from soil near biodiesel plant by culturing in mineral salt medium containing glycerol. Growth of selected isolates in medium containing glycerol as a sole carbon source were monitored and compared with reference bacterial strains. It was observed that 2 isolates grew better than reference strains according to optical density value at 600 nm. Both isolates were identified as Klebsiella pneumonia by 16S rRNA gene sequencing. The isolate 5-3, renamed as K. pneumonia KKU5 (Kp KKU5) was used for further study. The dhaT gene of Kp KKU5 shared 99 percent identity with those of K. pneumoniae (CP025630.1). To examine the ability of Kp KKU5 as a 1,3-propanediol producer, its fermentation broth containing glycerol was analyzed by HPLC. Under batch fermentation condition with 20 g/L initial glycerol, Kp KKU5 produced 0.375 g/L of 1,3-propanediol after 7 days. Despite its low production of 1,3-propanediol, we have demonstrated that Kp KKU5 was able to utilize glycerol as a sole carbon source. This study might provide some useful information to contribute in the research area of 1,3-propanediol production, which adds value to waste glycerol from biodiesel production.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2021

Modified: 2023-06-09

Issued: 2023-06-09

BibliograpyCitation : In Thai Society for Biotechnology. Thai Society for Biotechnology and International Conference Online (TSB Online 2021) (pp.641-651). Bangkok : Thai Society for Biotechnology

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