Fabrication and characterization of poly (3-Hydroxybutyrate-co-4-Hydroxybutyrate)/silk sericin films for wound healing applications

Name: Chanaporn Trakunjae

Organization : Universiti Sains Malaysia. School of Biological Sciences

Name: Hua Tiang Tan

Organization : Universiti Sains Malaysia. School of Biological Sciences

Name: Pilanee Vaithanomsat

Organization : Kasetsart University. Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI)

Name: Bakiah Shaharuddin

Organization : Universiti Sains Malaysia. Advanced Medical and Dental Institute

Name: Kumar Sudesh

Organization : Universiti Sains Malaysia. School of Biological Sciences

ThaSH: Sericin

ThaSH: Fibroblasts

ThaSH: Biopolymers

ThaSH: Plaster (Pharmacy)

ThaSH: Wound healing

Abstract: Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer (P34HB) is one of the copolymers in the polyhydroxyalkanoates (PHAs) family, which is an attractive candidate in the biomedical applications field owing to its excellent mechanical properties, biocompatibility and biodegradability. Also, silk sericin (SS) has been demonstrated to be immunologically inert due to its biocompatibility and various biological potentials. In this study, the P34HB copolymer produced by a newly engineered strain of Cupriavidus necator Re2058/pSK01/phaCcs, was fabricated with silk sericin by film casting technique. The developed polymer film was evaluated for its physical, mechanical, and wound healing properties. The P34HB incorporated with silk sericin films were found to be more hydrophilic in comparison with pristine P34HB film. The results proved that the better hydrophilicity of silk sericin could potentially overcome the hydrophobicity limitations of P34HB polymers. Furthermore, the addition of silk sericin contributes to the improved elongation at break, thus decreasing mechanical strength. In vitro study demonstrated that the Human Dermal fibroblasts (HDF) cultured on the high silk sericin-containing films (P34HB/SS, 1:3) exhibited better proliferation compared to those on films without silk sericin (P34HB). We propose, therefore, that P34HB/SS films have significant potential as wound dressing materials.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2021

Modified: 2023-05-29

Issued: 2023-05-29

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BibliograpyCitation : In Thai Society for Biotechnology. Thai Society for Biotechnology and International Conference Online (TSB Online 2021) (pp.294-301). Bangkok : Thai Society for Biotechnology

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