Antibacterial profiling of endophytic fungi sourced from Justicia betonica, a medicinal plant from the secluded Western Ghats region

Name: Parashiva J

Address: 333 Moo1, Thasud, Muang, Chiang Rai 57100

Organization : Center of Excellence in Fungal Research, Mae Fah Luang University

Email : ask.library@mfu.ac.th

Name: Nuthan BR1

Name: Rakshith D

Name: Satish S

keyword: Agar plug diffusion assay

ThaSH: Fungi

; Antibacterial activity

ThaSH: Antibacterial agents

; Diversity indices

ThaSH: Endophytic fungi

; Fungal endophytes

ThaSH: Xylariaceae

; Xylaria sp.

Abstract: The present study focused on exploring the endophytic fungal diversity associated with an ethnomedicinally valued plant, Justicia betonica located in the Western Ghats region of Karnataka and evaluation of their antibacterial potential. A total of 210 endophytic fungal isolates were obtained from healthy leaf and stem tissues. The isolates were grouped into 17 distinct genera, including morphospecies. The Colletotrichum is one of the most predominant genera representing 18.1% of the total isolates, followed by Cladosporium (14.8%), Pestalotiopsis (7.14%), and Alternaria (6.7%). The Shannon-wiener diversity (H) showed that fungal diversity in both plant tissues does not have significant differences. The antibacterial potential of the isolates was evaluated using a primary agar plug diffusion technique in which 28 isolates showed prominent antibacterial activity. Nine isolates that showed the highest antibacterial activity were subjected to secondary antibacterial screening using ethyl acetate extracts. The extracts showed varying inhibitory activity against both the pathogens Escherichia coli and Staphylococcus aureus with inhibition zone ranging from 14 to 32 mm. A Xylaria sp. designated as PJJBLF53 displayed the maximum inhibition zone against both the pathogens tested when compared to the standard antibiotic, gentamicin. The study indicates that J. betonica harbors diverse endophytic fungi that possess potent antibacterial potential, which could be investigated further for the isolation of novel antibacterial compounds.

Mae Fah Luang University. Learning Resources and Educational Media Center

Address: CHIANG RAI

Email: library@mfu.ac.th

Modified: 2022-11-02

Issued: 2022-11-02

บทความ/Article

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Asian Journal of Mycology (AJOM), Vol.4 No.2 (2021): p42-55

eng

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