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Nadda Muhamad. Atractylodin-loaded PLGA nanoparticles and cytotoxic effects in cholangiocarcinoma cells. Master's Degree(Biomedical Sciences). Thammasat University. Thammasat University Library. : Thammasat University, 2017.
Atractylodin-loaded PLGA nanoparticles and cytotoxic effects in cholangiocarcinoma cells
Name: Nadda Muhamad
keyword:
Atractylodin
Abstract:
The anti-cholangiocarcinoma activity of atractylodin, a major natural active constituent of the rhizomes of Atractylodes lancea (Thunb.) DC, has been demonstrated in a series of studies. However, this compound has poor water solubility and needs to be dissolved in organic solvent before used. Poly (lactic-co-glycolic acid) (PLGA) is currently extensively developed as polymeric nanoparticles for delivering hydrophobic drugs and enhancing its solubility in water. This present study aimed to develop atractylodin-loaded PLGA nanoparticles (AL-loaded PLGA NPs) and investigate in vitro cytotoxic activity against cholangiocarcinoma cell lines. The nanoparticle formulations were prepared using solvent displacement method. The encapsulation and loading efficiency were characterized and particle size, and zeta potential were determined by dynamic light scattering technique. Drug release was assessed in vitro. All NPs were found to be freely dispersible in water without aggregation. The size (mean+SD of diameter) of the prepared AL-loaded PLGA NPs using Resomer® RG502 and Resomer® RG504 were 158.33±0.21 nm and 161.27±1.87 nm, respectively with narrow size distribution. The zeta potential values of both nanoparticle formulations were observed to be lower than -20 mV which would facilitate stability of NPs. The % encapsulation efficiency (%EE) and % loading efficiency (%LE) of AL-loaded PLGA NPs using Resomer® RG502 were 52.02±1.64% and 2.30±0.07%, respectively and found to be significantly higher than that of using Resomer® RG 504. Drug release from AL-loaded PLGA NPs both formulations were observed up to 88% in 72 hours with biphasic manner. AL-loaded PLGA NPs using Resomer® RG502 were selected to study the stability and to investigate in vitro cytotoxic activity against cholangiocarcinoma cell lines, CL-6 and HuCCT-1. The best condition for storage of NPs was found to be stored in ultrapure water at 4 °C. For the in vitro cytotoxic activity, AL-loaded PLGA NPs were concentration- and time-dependent. The IC50 (mean+SD) of AL-loaded PLGA NPs at 72 hours were 38.28±2.62 µg/ml on CL-6 and 48.08±1.07 µg/ml on HuCCT-1 which were lower than that of atractylodin dissolved with water (IC50 ; mean+SD: 42.83±2.97 µg/ml and 63.71±3.90 µg/ml, respectively). Moreover, the toxicity on normal cell line, OUMS-36T-1F, of AL-loaded PLGA NPs were not found to be different from atractylodin dissolved with water. Besides, the blank PLGA NPs were observed to be non-toxic to both cholangiocarcinoma and normal cell lines. From these findings, the AL-loaded PLGA NPs were successfully developed and had potential to be used as drug delivery systems for the treatment of cholangiocarcinoma.
Thammasat University. Thammasat University Library
Address:
BANGKOK
Email:
preserv@tu.ac.th
Role:
advisor
Name: Kesara Na-Bangchang
Role:
co-advisor
Name: Chuda Chittasupho
Role:
co-advisor
Created:
2017
Modified:
2021-05-13
Issued:
2021-05-13
วิทยานิพนธ์/Thesis
application/pdf
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biomedical Sciences
Grantor: Thammasat University
©copyrights Thammasat University
RightsAccess:
ใช้เวลา
0.036286 วินาที
Nadda Muhamad
| Title | Contributor | Type |
|---|---|---|
| Atractylodin-loaded PLGA nanoparticles and cytotoxic effects in cholangiocarcinoma cells
มหาวิทยาลัยธรรมศาสตร์ Nadda Muhamad | Tullayakorn Plengsuriyakarn Kesara Na-Bangchang Chuda Chittasupho | วิทยานิพนธ์/Thesis |
Tullayakorn Plengsuriyakarn
| Title | Creator | Type and Date Create |
|---|---|---|
| Atractylodin-loaded PLGA nanoparticles and cytotoxic effects in cholangiocarcinoma cells
มหาวิทยาลัยธรรมศาสตร์ Tullayakorn Plengsuriyakarn;Kesara Na-Bangchang;Chuda Chittasupho | Nadda Muhamad | วิทยานิพนธ์/Thesis |
| Anti-cholangiocarcinoma and toxicity evaluation of Atractylodes lancea (Thunb.) DC. and Kaempferia galanga Linn. herbal extract preparations for drug development
มหาวิทยาลัยธรรมศาสตร์ Kesara Na-bangchang;Tullayakorn Plengsuriyakarn | Limeneh, Asmare Amuamuta | วิทยานิพนธ์/Thesis |
Kesara Na-Bangchang
Chuda Chittasupho
| Title | Creator | Type and Date Create |
|---|---|---|
| Development of peptide conjugated nanoparticles as drug delivery system for specific targeting to leukemia cell lines
มหาวิทยาลัยเชียงใหม่ Chuda Chittasupho;Chadarat Ampasavate;Siriporn Okonogi;Songyot Anuchapreeda | Rungsinee Phongpradist | วิทยานิพนธ์/Thesis |
| Effects of partially purified fractions of kaffir lime (Citrus hystrix DC.) leaf on cell cycle in K562 and Molt4 leukemic cell lines
มหาวิทยาลัยเชียงใหม่ Chuda Chittasupho;Songyot Anuchapreeda;Siripogn Okqrogi;Chadarat Ampasavate;Sawitree Chiampanichayakul) | Wipavee Utthawang | วิทยานิพนธ์/Thesis |
| Atractylodin-loaded PLGA nanoparticles and cytotoxic effects in cholangiocarcinoma cells
มหาวิทยาลัยธรรมศาสตร์ Tullayakorn Plengsuriyakarn;Kesara Na-Bangchang;Chuda Chittasupho | Nadda Muhamad | วิทยานิพนธ์/Thesis |
| Development of Ketoconazole nanoparticle in situ Ophthalmic gel preparation
มหาวิทยาลัยเชียงใหม่ Chuda Chittasupho;Jutamas Jiaranaikulwanitch | Chutima Chaiwut | วิทยานิพนธ์/Thesis |