Abstract:
The objective of this study were to prepare Centella asiatica (CA) leaves extract by using 2 methods: squeezing (following Thai wisdom) and solvents extraction (2 solvents: DI water and ethanol) and to investigate the bioactive compound (total phenolic: TPC, flavonoid: TFC and proanthocyanidin TPAC contents) and antioxidant activity (DPPH, ABTS and FRAP activities) of these Centella asiatica extracts. Results showed that the DI water CA extract had the highest TPC (262.5±1.1 mg GAE/g sample). In addition, the antioxidant capacity of these CA extracts tended to be greatest on DI water extracts (DPPH 1503.0±19.3, ABTS 1863.0±18.3 and FRAP 4760.2±56.0mg TEAC/g sample). For the development of base hair tonic, the best base formula composed of acrylate copolymer (0.4 % w/w in formula) that its characteristic was clearly liquid, smooth hair tonic. Moreover, hair tonic with Centella asiatica ethanolic extract was developed in CA concentration 10 % w/w in formula that has stability and high customer stratification (3.8±0.8 of total 5 scores) without irritation. Furthermore, the result of the efficiency of CA hair tonic. Showed that this CA product could be anti-hair loss (anti-hair fall~ 26.8 ± 7.0% n=30). The results suggested that Centella asiatica extract (especially ethanolic extraction) may be used as natural active ingredient for hair nourish and anti-hair loss product.