Lipids and carotenoids productions from oleaginous yeasts using rice residue from food waste as a carbon source

การผลิตลิพิดและแคโรทีนอยด์จากโอลิจินัสยีสต์โดยใช้เศษข้าวที่ได้จากขยะอาหารเป็นแหล่งคาร์บอน

Name: Wimada Srisuwan

ThaSH: Lipids

Classification :.DDC: 572.57

ThaSH: Carotenoids

Abstract: Rice residue from food waste composed of starch as a main component which can be either hydrolyzed to be fermentable sugars or directly used as a carbon source for growth and high value metabolite production by various types of microorganisms. Therefore, this study aimed to investigate the utilization of rice residue and rice residue hydrolysate from food waste as a carbon source for biomass, lipids and carotenoids productions by oleaginous red yeast. Rice residue was obtained from canteen of the Faculty of Agro-Industry, Chiang Mai University. It composed of moisture content (7.18±0.34%), crude fat (0.75±0.01%), crude protein (1.29±0.03%), ash content (0.38±0.03%) and carbohydrate content (90.00±0.01%), respectively. Rice residue was subjected to enzymatic hydrolysis using α-amylase (180 U/g substrate) and amyloglucosidase (AMG) (120 U/g substrate), resulting in the maximal reducing sugar of 168.02±0.02 g/L. The screening of oleaginous red yeast from flowers and leaves samples collected from Doi-Inthanon National Park, the culture collection of the Thailand Institute of Scientific and Technological Research (TISTR) and the Division of Biotechnology, Faculty of Agro-Industry, Chiang Mai University were studied. Sixty-seven isolates were obtained and only four isolates were identified as oleaginous red yeasts which could accumulate both of intracellular lipids (>20% w/w) and carotenoids. These oleaginous red yeasts were identified by a molecular genetics technique based on viii nucleotide sequence analysis of the D1/D2 domain of 26S rDNA. These yeasts were identified as Rhodotorula glutinis KM281508, Rhodosporidium sp. KM281510, Sporidiobolus pararoseus KX709872 and Diozegia sp. TISTR5792, respectively. These oleaginous red yeasts produced maximal volumetric production of lipids of 1.61±0.09, 1.70±0.09, 1.04±0.09 and 0.98±0.04 g/L, with lipids content of 24.26±0.56, 23.69±0.91, 23.07±0.80 and 22.43±1.09 % (w/w), respectively. These yeasts also accumulated carotenoids in their cells with volumetric production of total carotenoids of 0.82±0.05, 1.57±0.05, 2.68±0.09 and 1.64±0.35 mg/L and total carotenoids yield of 150±0.10, 280±0.22, 595±0.90 and 400±0.13 μg/g, respectively, when cultivated in the basal medium supplemented with enzymatic-rice residue hydrolysate as a carbon source. Sporidiobolus pararoseus KX709872 could grow well in the basal medium supplemented with rice residue (without hydrolysis) as a carbon source. Strain KX70982 produced the maximum volumetric production of lipids and lipids content of 1.26±0.01 g/L and 21.67±0.02% (w/w), respectively. While, the volumetric production of total carotenoids and total carotenoids yield were 0.64±0.00 mg/L and 110.0±0.00 μg/g, respectively. Moreover, strain KX709872 produced α-amylase (540±0.09 mU/mL) and AMG (23±0.00 mU/mL), and showed high ability in directly converting rice residue from food waste for biomass, lipids and carotenoids productions. The effects of medium composition on biomass, lipids and carotenoids productions of strain KX709872 were investigated using the Plackett-Burman design. Only one factor of the C:N ratio was significantly influenced on biomass, lipids and carotenoids productions at a confidence level above 90%. Optimal conditions for biomass, lipids and carotenoids production were further investigated using response surface methodology (RSM) via a central composite design (CCD). At a C:N ratio of 25:1, pH of 5.44, temperature of 25.34°C and agitation rate of 192.73 rpm, biomass, lipids, lipids content, lipids productivity, total carotenoids, β-carotene, total carotenoids yield and β-carotene yield reached 17.96±0.07 g/L, 8.99±0.33 g/L, 49.22±0.12% (w/w), 1.67±0.11 g/L/d, 6.81±0.06 mg/L, 2.71±0.08 mg/L, 387±14.11 μg/g and 154±8.53 μg/g, respectively. The production of lipids and carotenoids was investigated in a 5−L stirred tank bioreactor containing 2.5−L of optimized medium (agitation rate, 300 rpm; aeration ix rate, 3.0 vvm for 7 days). The maximum biomass, lipids, lipids content and lipids productivity were 16.33±0.49 g/L, 8.75±0.13 g/L, 56.61±0.04% (w/w) and 2.19±0.03 g/L/d achieved at day−4 of the cultivation period, with the maximum total carotenoids, β-carotene, total carotenoids yield and β-carotene yield of 7.10±0.09 mg/L, 2.99±0.06 mg/L, 600±0.06 μg/g and 252±0.01 μg/g achieved at day−6 of the cultivation period, respectively. Furthermore, oleic acid content of 62.13±0.04% was achieved. This result indicated that lipids from strain KX709872 show high potential as the third feedstock for biodiesel production because of the high oleic acid content. Moreover, this study demonstrated a one-step production of microbial lipids and carotenoids from starchy material using amylolytic oleaginous red yeast, which is easy to operate and low-cost.

Chiang Mai University Library

Address: Chiang Mai

Email: cmulibref@cmu.ac.th

Name: Punyawatt Pintathong

Role: Cairman

Name: Charin Techapun

Role: Member

Name: Thanongsak Chaiyaso

Role: Member

Name: Churairat Moukamnerd

Role: Member

Created: 2017

Modified: 2020-01-06

Issued: 2020-01-06

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Th 572.57 W757L

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biotechnology

Grantor: Chiang Mai University

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